DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis



Título del documento: DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis
Revue: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000398146
ISSN: 0717-3458
Autores: 1
1
2
5
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3
4
Instituciones: 1Instituto Nacional de Tecnología Agropecuaria, Instituto de Recursos Biológicos, Castelar, Buenos Aires. Argentina
2Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Bariloche, Bariloche, Río Negro. Argentina
3Instituto Nacional de Tecnologia Agraria y Alimentaria, Centro de Investigación Forestal, Madrid. España
4Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Instituto de Biotecnología, Castelar, Buenos Aires. Argentina
5Consejo Nacional de Investigaciones Científicas y Técnicas, La Plata, Buenos Aires. Argentina
Año:
Volumen: 16
Número: 2
Paginación: 1-12
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés Austrocedrus chilensis (D. Don) Pic. Ser. et Bizzarri commonly known as Patagonian cypress is a member of the Cupressaceae family, characterized by a high adaptive potential for growing in marginal areas and good timber quality. The species grows over a wide area and under a wide range of rainfall. This study assessed adaptive genetic variation at SNP level in candidate genes involved in response to drought stress. Results: A total of 18 single nucleotide polymorphisms (SNPs) were found among 1,428 bp. Average nucleotide diversi ty value ( π = 0.00312) was similar to those previously reported in other Cupressaceae. The Fst average among genes and populations was 0.163 and the lowest differentiation was observed in continuous and humid populations. A number of neutrality tests were applied to find evidence of positive selection in our candidate gene set, but only AcAQP2 gene in Pedregoso and San Ramón populations revealed significant departures from neutrality with positive values suggesting balancing selection. Conclusions: In this study we report the levels of nucleotide diversity searched in some drought stress candidate genes in Austrocedrus chilensis and the selective factors that may be acting on this species
Disciplinas: Biología
Palabras clave: Botánica,
Fisiología vegetal,
Genética,
Adaptación,
Sequía,
Variación genética,
Selección de equilibrio,
Ciprés,
Austrocedrus chilensis,
Polimorfismos de nucleótido único (SNPs)
Keyword: Biology,
Botany,
Genetics,
Plant physiology,
Adaptation,
Genetic variation,
Drought,
Balancing selection,
Cypress,
Austrocedrus chilensis,
Single nucleotide polymorphism (SNPs)
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