| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358925 |
| ISSN: | 0717-3458 |
| Autores: | Odisi, Estacio Jussie1 Silvestrin, Marcela Bruschi1 Takahashi, Rodrigo Yoji Uwamori1 Silva, Marcus Adonai Castro da1 Lima, Andre Oliveira de Souza1 |
| Instituciones: | 1Universidade do Vale do Itajai, Centro de Ciencias Tecnologicas, da Terra e do Mar, Itajai, Santa Catarina. Brasil |
| Año: | 2012 |
| Volumen: | 15 |
| Número: | 5 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | Cellulases and lipases have broad industrial application, which calls for an urgent exploration of microorganisms from extreme environments as valuable source of commercial enzyme. In this context, the present work describes the bioprospection and identification of deep-sea bacteria that produce cellulases and lipases, as well their optimal temperature of activity. Results: The first step of this study was the screening of cellulolytic and lipolytic deep-sea bacteria from sediment and water column, which was conducted with substrates linked with 4-Methylumbelliferyl. Among the 161 strains evaluated, 40 were cellulolytic, 23 were lipolytic and 5 exhibited both activities. Cellulolytic and lipolytic bacteria are more common in sediment than at the water column. Based on the ability to produce cellulases and lipases three isolates were selected and identified (16S rRNA sequencing) as Bacillus stratosphericus, B. aerophilus and B. pumilus. Lipases of strain B. aerophilus LAMA 582 exhibited activity at a wide temperature range (4º to 37ºC) and include psychrophilic behaviour. Strain Bacillus stratosphericus LAMA 585 can growth in a rich (Luria Bertani) and minimal (Marine Minimal) medium, and does not need an inducer to produce its mesophilic cellulases and lipases. Conclusions: Deep-sea sediments have great potential for bioprospection of cellulase and lipase-producing bacteria. The strains LAMA 582 and LAMA 585 with their special features, exhibit a great potential to application at many biotechnology process |
| Disciplinas: | Biología, Química |
| Palabras clave: | Bacterias, Biología acuática, Bioquímica, Biotecnología, Fondo marino, Océano Atlántico, Bioprospección, Enzimas, Celulasas, Lipasas, Explotación de recursos |
| Keyword: | Biology, Chemistry, Aquatic biology, Bacteria, Biochemistry, Biotechnology, Deep sea, Atlantic Ocean, Bioprospection, Cellulases, Lipases, Enzymes, Resource exploitation |
| Texte intégral: | Texto completo (Ver HTML) |
