| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000266086 |
| ISSN: | 0717-3458 |
| Autores: | Hosseini, Ramin-Mulligan, Bernard J1 |
| Instituciones: | 1University of Nottingham, School of Biological Sciences, Nottingham, Nottinghamshire. Reino Unido |
| Año: | 2002 |
| Periodo: | Abr |
| Volumen: | 5 |
| Número: | 1 |
| Paginación: | 13-14 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | Changes in the DNA content and organisation of senescing rice cell cultures (Taipei 309) were studied, using PCR and Southern blot analyses. A mitochondrial gene (coxII), a plastid gene (psaA) and a nuclear DNA maker (RG64) were analysed. The amplification of mitochondrial (mt), plastid and nuclear DNA produced the expected fragments, indicating that there were still some intact organelles and nuclei in the senescing rice cells. However, in plastid and nuclear DNA, changes in the number and size of the PCR products were observed. Southern blot analysis revealed that mt and nuclear DNA contents declined, but plastid DNA content remained relatively the same throughout the senescence |
| Disciplinas: | Agrociencias |
| Palabras clave: | Gramíneas, Biotecnología, Genética, Arroz, Oryza sativa, Muerte celular programada, ADN |
| Keyword: | Agricultural sciences, Gramineae, Oryza sativa, Programmed cell death, DNA, Rice, Genetics |
| Texte intégral: | Texto completo (Ver HTML) |
