| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358914 |
| ISSN: | 0717-3458 |
| Autores: | Pérez de la Torre, Mariana1 García, Martín2 Heinz, Ruth2 Escandón, Alejandro3 |
| Instituciones: | 1Instituto Nacional de Tecnología Agropecuaria, Instituto de Floricultura, Buenos Aires. Argentina 2Instituto Nacional de Tecnología Agropecuaria, Instituto de Biotecnología, Buenos Aires. Argentina 3Instituto de Genética Ewald A. Favret, Castelar, Buenos Aires. Argentina |
| Año: | 2012 |
| Volumen: | 15 |
| Número: | 5 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5CT, 5CA, 5GA, 5GACA, 3CAC, 3TG and 3TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5GACA and 3AG primers, respectively, while the average values for MI and PIC were 0.367 and 0.231, respectively. The more informative primers were 5GACA and 5GA, and the less informative was 3AC. Simple matching coefficient of similarity varied from 0.8875 to 0.6659, indicating high levels of genetic similarity among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters; one comprised genotypes of the five departments, while the second included individuals from Guaraní and Oberá regions and the third cluster included the San Pedro individuals. The overall grouping pattern is in agreement with principal coordinate analysis (PCoA). Conclusions: The Bayesian cluster analysis revealed structuring of the C. caesia population and two clusters were identified, which correspond to UPGMA major clades. The AMOVA test for all populations showed highest genetic variation within populations (90%), meanwhile the Fst coefficient was 0.098, indicating a medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among populations. In this work the use of thirteen ISSR markers, allowed the c |
| Disciplinas: | Agrociencias, Biología |
| Palabras clave: | Plantas ornamentales, Genética, Calibrachoa caesia, Solanaceae, Biología molecular, Biotecnología, Variabilidad genética, Marcadores moleculares, Microsatélites |
| Keyword: | Agricultural sciences, Biology, Ornamental plants, Genetics, Calibrachoa caesia, Solanaceae, Molecular biology, Biotechnology, Genetic variability, Molecular markers, Microsatellites |
| Texte intégral: | Texto completo (Ver HTML) |
