| Revue: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451932 |
| ISSN: | 1984-8250 |
| Autores: | Zhang, Jie1 Tong, Chuan Feng1 Wan, Jing1 Wang, Yang Gan1 |
| Instituciones: | 1Wuhan University, Zhongnan Hospital, Zhongnan, Wuhan. China |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Xuezhikang (XZK) is an extract of Chinese red yeast rice. It has multiple protective effects in cardiovascular systems. However, the underlying mechanism by which XZK affects free fatty acid (FFA)-induced lipogenesis in hepatocellular steatosis model is still unknown. Herein, we investigated this mechanism in HepG2 cells. The HepG2 cells were treated with palmitate acid (PA) to induce lipogenesis. Then the PA-induced HepG2 cells were subsequently treated with XZK. After 24 h of treatment, we determined the intracellular triglyceride (TG) contents and average areas of lipid droplets. To study the involvement of AMPK signaling pathway, we pre-treated the PA-induced HepG2 cells with Compound C, an AMPK inhibitor, before XZK treatment. Expressions of p-AMPK and AMPK were determined by Western blot. The results showed that XZK decreased TG content and lipid accumulation in hepatocellular steatosis model. Compound C abolished the effects of XZK. These results demonstrated for the first time that XZK protects hepatocytes against lipid accumulation induced by free fatty acids. Its effects may be mediated by the activation of AMPK pathway |
| Disciplinas: | Química, Biología |
| Palabras clave: | Bioquímica, Farmacología, Plantas medicinales, Esteatosis hepatocelular, AMPK, Acumulación de lípidos |
| Keyword: | Biochemistry, Pharmacology, Medicinal plants, Hepatocellular steatosis, AMPK, Lipid accumulation |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
