Revue: | Brazilian Journal of Pharmaceutical Sciences |
Base de datos: | PERIÓDICA |
Número de sistema: | 000451809 |
ISSN: | 1984-8250 |
Autores: | Matos, Natália Alves de1 Reis, Diego Carlos dos2 Rocha, Lucas Kraemer3 Mattos, Matheus Silvério de3 Cassali, Geovanni Dantas2 Russo, Remo Castro3 Perez, Andrea de Castro4 Klein, André4 |
Instituciones: | 1Universidade Federal de Ouro Preto, Instituto de Ciencias Exatas e Biologicas, Ouro Preto, Minas Gerais. Brasil 2Universidade Federal de Minas Gerais, Departamento de Patologia Geral, Belo Horizonte, Minas Gerais. Brasil 3Universidade Federal de Minas Gerais, Departamento de Fisiologia e Biofisica, Belo Horizonte, Minas Gerais. Brasil 4Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas, Belo Horizonte, Minas Gerais. Brasil |
Año: | 2022 |
Volumen: | 58 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | Protease-activated receptors (PARs) are metabotropic G-protein-coupled receptors that are activated via proteolytic cleavage of a specific sequence of amino acids in their N-terminal region. PAR2 has been implicated in mediating allergic airway inflammation. This study aims to study the effect of PAR2 antagonist ENMD1068in lung inflammation and airway remodeling in experimental asthma. Allergic lung inflammation was induced in sensitized BALB/c mice through intranasal instillations of ovalbumin (OVA), and mice were pretreated with ENMD1068 1 hour before each OVA challenge. Bronchoalveolar lavage fluid (BALF) was collected, and the lungs were removed at different time intervals after OVA challenge to analyze inflammation, airway remodeling and airway hyperresponsiveness. Ovalbumin promoted leukocyte infiltration into BALF in a PAR2-dependent manner. ENMD1068 impaired eosinophil peroxidase (EPO) and myeloperoxidase (MPO) activity in the lung parenchyma into BALF and reduced the loss of dynamic pulmonary compliance, lung resistance in response to methacholine, mucus production, collagen deposition and chemokine (C-C motif) ligand 5 expression compared to those in OVA-challenged mice. We propose that proteases released after an allergen challenge may be crucial to the development of allergic asthma in mice, and PAR2 blockade may be useful as a new pharmacological approach for the treatment of airway allergic diseases |
Disciplinas: | Medicina |
Palabras clave: | Medicina experimental, Farmacología, Neumología, Bloqueo farmacológico, Receptores químicos, Proteasas, Alergia respiratoria, Asma, Inflamación, Pulmones |
Keyword: | Experimental medicine, Pharmacology, Pneumology, Pharmacological blockade, Chemical receptors, Proteases, Respiratory allergy, Asthma, Lungs, Inflammation |
Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |