| Revue: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451079 |
| ISSN: | 1984-8250 |
| Autores: | Chiamenti, Lisandra1 Silva, Francini Pereira da2 Schallemberger, Karoline Demoliner, Meriane3 Rigotto, Caroline4 Fleck, Juliane Deise4 |
| Instituciones: | 1Universidade Feevale, Programa de Pos-Graduacao em Toxicologia e Analisis Toxicologico, Novo Hamburgo, Rio Grande do Sul. Brasil 2Universidade Feevale, Programa de Pos-Graduacao em Virologia, Novo Hamburgo, Rio Grande do Sul. Brasil 3Universidade Feevale, Programa de Pos-Graduacao em Qualidade Ambiental, Novo Hamburgo, Rio Grande do Sul. Brasil 4Universidade Feevale, Laboratorio de Microbiologia Molecular, Novo Hamburgo, Rio Grande do Sul. Brasil |
| Año: | 2019 |
| Volumen: | 55 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Cymbopogon citratus and C. nardus are noteworthy among the several existing plant species displaying medicinal properties, due to the potential pharmacological activity of these species, including antiviral, antibacterial, antifungal and anti-trypanosomal activities. The objective of this study was to carry out in vitro toxicity tests of plant extracts from both species and analyze potential antiviral activity against Human mastadenovirus serotype 5 (HAdV-5). Two cell lines (A549 and VERO) were used and mitochondrial and lysosomal viability were determined by the MTT and neutral red assay, respectively, after two exposure times (24 hours and six days). The aim of these assays was to counteract the behavior of the extracts against the different cell lines and determine their non-toxic concentration range, in order to evaluate possible antiviral activity against HAdV-5. Plaque reduction and inhibition index of viral titer assays were performed using the maximum non-cytotoxic concentrations (MNCC) of each extract. The results indicate MNCC at 625 μg/mL for all extracts, except for Cymbopogon nardus obtained with 80% ethanol (CN80), which showed toxicity at concentrations higher than 312.5 μg/mL. CN80 was the only extract that displayed potential activity against HAdV-5, at a concentration of 75 μg/mL, becoming a candidate for extract fraction purification and/or the isolation of substances related to the observed antiviral activity |
| Disciplinas: | Química |
| Palabras clave: | Fitoquímica, Plantas medicinales, Actividad antiviral, Mastadenovirus humano, Cymbopogon citratus, Cymbopogon nardus, Poaceae |
| Keyword: | Phytochemistry, Medicinal plants, Antiviral activity, Human mastadenovirus, Cymbopogon citratus, Cymbopogon nardus, Poaceae |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
