Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis
Título del documento: Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis
Revue: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451434
ISSN: 1984-8250
Autores: 1
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Instituciones: 1University of Medicine and Pharmacy, Faculty of Pharmacy, Tirgu Mures. Rumania
Año:
Volumen: 56
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés The present work describes the development of a capillary electrophoresis (CE) method for the chiral discrimination of amlodipine (AML) enantiomers using cyclodextrine (CD) derivatives as chiral selectors. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector; and carboximethyl-β-CD (CM-β-CD) was selected for the enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors were varied at the same time to optimize the separation method. The optimized method (25 mM phosphate buffer, pH = 9.0, 15 mM CM-β-CD, 15 ºC, + 25 kV, 30 mbar/1 second, detection wavelength 230 nm) was successfully applied for the baseline separation of AML enantiomers within 5 minutes. Successful validation and application of the proposed CE method suggest its routine use in enantioselective control of AML in pharmaceutical preparations
Disciplinas: Química
Palabras clave: Química farmacéutica,
Química analítica,
Amlodipina,
Separación selectiva,
Quiralidad,
Electroforesis por capilaridad,
Ciclodextrina
Keyword: Medicinal chemistry,
Analytical chemistry,
Selective separation,
Amlodipine,
Capillary electrophoresis,
Chirality,
Cyclodextrin
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