Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesion
Título del documento: Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesion
Revue: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451726
ISSN: 1984-8250
Autores: 1
2
2
1
2
1
1
Instituciones: 1University Mustapha Stambouli of Mascara, Faculty of Nature and Life Sciences, Mascara. Argelia
2Universidade Federal do Vale do Sao Francisco, Central de Analisis de Drogas, Medicamentos e Alimentos, Petrolina, Pernambuco. Brasil
Año:
Volumen: 58
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés This study assessed the inhibitory potential of the probiotics Lactobacillus (LB) exopolysaccharides (EPS) with or without extracts of Satureja calamintha on enteropathogenic Escherichia coli (EPEc) responsible for gastroenteritis. Methanolic and hydromethanolic extracts were prepared by cold maceration and subjected to phytochemical screening. The compounds of the extracts were determined with the colorimetric assays and identified using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Antioxidant activities of the extracts were also evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging. Antibacterial effect on EPEc was evaluated by using both agar disc diffusion and microdilution methods. The in vitro test of auto-aggregation was investigated. Microbiological analysis showed that 63% of the isolated LB were producing EPS, with the amount ranging from 8.21 to 43.13 mg/L. Chemical analysis of the extracts revealed the presence of polyphenols and flavonoids, more abundant in the hydromethanolic extract, which presented the highest content with 2.11 mg EGA/g of polyphenol and 1.64 mg EC/g of flavonoids and 1.71 mg EGA/g of polyphenol and 1.15 mg EC/g of flavonoids in the methanolic extract. Hydromethanolic extracts and EPS exhibited a more important activity than did the methanolic extract against EPEc. The combined action of EPS and extracts reduced the aggregation ability of EPEc and decreased the rate of their adhesion
Disciplinas: Medicina
Palabras clave: Microbiología,
Plantas medicinales,
Probióticos,
Bacterias enteropatógenas,
Satureja calamintha,
Lamiaceae,
Lactobacillus,
Exopolisacáridos,
Escherichia coli
Keyword: Microbiology,
Medicinal plants,
Probiotics,
Enteropathogenic bacteria,
Satureja calamintha,
Lamiaceae,
Lactobacillus,
Exopolysaccharides,
Escherichia coli
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