| Revue: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000452174 |
| ISSN: | 1984-8250 |
| Autores: | Parvizi, Mahdieh1 Farzanefar, Saeed2 Khalaj, Ali1 Tafakhori, Abbas3 Daha, Fariba Johari4 Saffar, Hana3 Sadeghzadeh, Masoud4 Naseri, Maryam2 Ahmadzadehfar, Hojjat5 Abbasi, Mehrshad2 |
| Instituciones: | 1Tehran University of Medical Sciences, Faculty of Pharmacy, Teherán. Irán 2Tehran University of Medical Sciences, Vali-Asr Hospital, Teherán. Irán 3Tehran University of Medical Sciences, Imam Khomeini Hospital, Teherán. Irán 4Nuclear Science and Technology Research Institute, Radiation Application Research School, Teherán. Irán 5University Hospital Bonn, Department of Nuclear Medicine, Bonn, Nordrhein-Westfalen. Alemania |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | There is no biodistribution or imaging data on 99mtechnetium (Tc)-hexamethyl propylamine oxime (HMPAO)-labeled platelets in the literature. The current study aimed to present updated information about the clinical procedures for preparation and use of labeled platelets. Following two-step centrifugation at 1500 and 2500 rpm, the platelets were extracted from whole blood into platelet-rich plasma (PRP) above the buffy coat and then from PRP into a platelet pellet at the bottom of the tube. The 99mTc-HMPAO-labeled platelets were inspected for purity, viability, release of 99mTc from platelets, and sterility. Also, microscopic examination and thin layer chromatography (TLC) were performed. Biodistribution was assessed following necropsy in BALB/c mice and through imaging of New Zealand rabbits. The separation ratio was estimated at 98%, and radiochemical purity was measured to be 80%. The labeling efficiency was above 30% in more than half of the assays (range: 17-43%). The release of 99mTc from platelets was 9% per hour at 37ºC. After 24 hours, stability was estimated at 54% in the human serum. The target organs of mice included the spleen and liver. In rabbits, the imaging results indicated liver as the target organ. Thyroid uptake was negligible up to 90 minutes. Based on the findings, extraction of platelets and labeling them with 99mTc-HMPAO is a feasible and safe approach in routine practice |
| Disciplinas: | Química, Medicina |
| Palabras clave: | Farmacología, Diagnóstico, Radiomarcaje, Tecnecio 99, Biodistribución, Control de calidad |
| Keyword: | Pharmacology, Diagnosis, Radiolabelling, Technetium 99, Biodistribution, Quality control |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
