| Revue: | Brazilian journal of microbiology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000291099 |
| ISSN: | 1517-8382 |
| Autores: | Rodrigues, Cristina Crespo1 Hofling, Jose Francisco Boriollo, Marcelo Fabiano Gomes Rodrigues, Janaina Aparecida de Oliveira Azevedo, Ricardo Antunes de2 Goncalves, Reginaldo Bruno Gomes, Luiz Humberto Tavares, Flávio Cesar Almeida |
| Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Odontologia, Piracicaba, Sao Paulo. Brasil 2Universidade de Sao Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Piracicaba, Sao Paulo. Brasil |
| Año: | 2004 |
| Periodo: | Ene-Jun |
| Volumen: | 35 |
| Número: | 1-2 |
| Paginación: | 40-47 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | The aim of the present research was to evaluate the protein polymorphism degrees among forty-eight C. albicans isolates from fourteen anatomical sites of clinical patients by polyacrylamide gel electrophoresis (SDS-PAGE) and numerical analyzes, in order to identify subspecies and their similarities in some infectious niches. Cell cultures were grown in YEPD medium, collected by centrifugation, and washed in cold saline solution. The whole-cell proteins were extracted by cell disruption using glass beads and submitted to SDS-PAGE technique. After electrophoresis, the protein bands were stained with coomassie-blue and analyzed by statistics package NTSYS-pc version 1.70 software. Similarity matrixes and dendrograms were generated by application of similarity coefficient of simple matching and UPGMA algorithm, respectively. The results obtained showed several C. albicans subtypes and their similarity degrees (80% to 100%). Such data showed that same patients may be infected by two or more C. albicans subtypes in certain anatomical sites (i.e. only in oral cavity of immunocompromised patients, blood, or tracheal secretion), or yet, two or more patients can be infected in identical anatomical sites (i.e. bronchial washing, urine, oral cavity, tracheal secretion, vaginal secretion, and healthy saliva) with a same C. albicans subtype. However, two or more patients also can show infections in corresponding sites (i.e. oral cavity of immunocompromised patients, blood, oropharyngeal secretion, oral cavity, tracheal secretion, vaginal secreti |
| Disciplinas: | Medicina |
| Palabras clave: | Odontología, Parasitología, Candida albicans, Electroforesis, Proteínas, Análisis numérico |
| Keyword: | Medicine, Dentistry, Parasitology, Candida albicans, Electrophoresis, Proteins, Numerical analysis |
| Texte intégral: | Texto completo (Ver HTML) |
