| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000308643 |
| ISSN: | 0104-6632 |
| Autores: | Piza, F.A.T1 Siloto, A.P Carvalho, C.V Franco, T.T |
| Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Engenharia Quimica, Campinas, Sao Paulo. Brasil |
| Año: | 1999 |
| Periodo: | Jun |
| Volumen: | 16 |
| Número: | 2 |
| Paginación: | 185-192 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | A culture medium for a wild strain of Bacillus cereus was developed for chitosanase production by using an experimental design. The factors having the strongest influence on chitosanase production were ammonium sulfate concentration, aeration, pH and the interaction between the first two parameters. Optimal conditions for chitosan hydrolysis were pH 5.8 and 54 oC; however, hydrolysis activity drastically decreased at pH 7.0. The enzyme was purified (single-electrophoretic band) by partitioning in an aqueous two-phase system (ATPS), followed by cation-exchange chromatography with a 66% yield. Chitosanase was mainly collected in the top phase (K = 129) of a 22% PEG 1,500, 13% phosphate (pH = 5.8) and 12% NaCl (w/w) solution, and the main protein contaminants were evenly distributed between the phases (K = 1.07). The apparent molecular weight and the isoelectric point of the chitosanase, determined by SDS-PAGE electrophoresis and by isoelectric focalization, were 47 kDa and 8.8, respectively |
| Disciplinas: | Química |
| Palabras clave: | Bioquímica, Ingeniería química, Sistema acuoso bifásico, Quitosanasas, Diseño experimental factorial, Bacillus cereus |
| Keyword: | Chemistry, Biochemistry, Chemical engineering, Aqueous two-phase system, Chitosanases, Factorial experimental design, Bacillus cereus |
| Texto completo: | Texto completo (Ver HTML) |
