| Revue: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000365588 |
| ISSN: | 1516-8913 |
| Autores: | Silveira, Daniela Garcia1 Lino, Lucimeire Souza Morais2 Souza, Antonio da Silva2 Souza, Fernanda Vidigal Duarte2 |
| Instituciones: | 1Instituto Federal Baiano, Guanambi, Bahia. Brasil 2Empresa Brasileira de Pesquisa Agropecuaria, Mandioca e Fruticultura Tropical, Cruz das Almas, Bahia. Brasil |
| Año: | 2013 |
| Periodo: | Jul-Ago |
| Volumen: | 56 |
| Número: | 4 |
| Paginación: | 547-556 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Neoglaziovia variegata (Arruda) Mez, a Bromeliad endemic to the Brazilian Caatinga and source of fiber for many different products, is considered an endangered species. Leaf and stem segments were cultivated on Murashige and Skoog (MS) medium with combinations of 2,4- dichlorophenoxyacetic acid (2,4-D) (0, 9, 18 and 27 µM) and glutamine (0, 50, 100, 150, 200 mg L-1) for the induction of embryogenic calli. Leaf segments did not present a favorable response for the combinations analyzed. The best response for embryogenic calli frequency with the best distribution of somatic embryos on the surface and better conversion rate was obtained using the stems explants in the medium containing 18 µM 2,4-D and 100 mg L-1 glutamine. The embryogenic calli obtained from the different induction media were transferred to MS medium supplemented with 30 g.L-1 sucrose, 2.4 g.L-1 Phytagel®, 0.53 µM α-naphthalene acetic acid (NAA) and 0.88 µM of 6-benzylaminopurine (BAP). The regenerated plants presented normal growth; however, many embryos did not fully develop |
| Disciplinas: | Biología, Agrociencias |
| Palabras clave: | Angiospermas, Fisiología vegetal, Propagación vegetativa, Cultivo de tejidos, Embriogénesis somática, Auxinas, Neoglaziovia variegata, Bromeliaceae |
| Keyword: | Biology, Agricultural sciences, Angiosperms, Plant physiology, Vegetative propagation, Tissue culture, Somatic embryogenesis, Auxins, Neoglaziovia variegata, Bromeliaceae |
| Texte intégral: | Texto completo (Ver HTML) |
