| Revue: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000365605 |
| ISSN: | 1516-8913 |
| Autores: | Portes, Ciro da Silva1 Oliveira, Andrielen Virke de1 Simer, Patricia1 Lunkes, Alessandra Machado1 Coelho, Alexandre Rodrigo1 |
| Instituciones: | 1Universidade Tecnologica Federal do Parana, Francisco Beltrao, Parana. Brasil |
| Año: | 2013 |
| Periodo: | Jul-Ago |
| Volumen: | 56 |
| Número: | 4 |
| Paginación: | 619-628 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | This work evaluated the antagonism of killer positive yeast strains (isolated from 11 samples of different frozen fruit pulps) against the strains of Penicillium expansum and Aspergillus ochraceus. Of the total 41 killer yeasts tested in YM agar, 19 showed antibiosis against P. expansum and A. ochraceus, with inhibition zone ranging from 10 to 18 mm and 10 to 19 mm, respectively. In the following step, the extracellular activity of Kluyveromyces sp. FP413 was tested performing the assay in YM broth. The antifungal activity of Kluyveromyces sp. FP413 cell-free culture supernatant (25oC/96 h) was more effective against the conidia germination, showing inhibition rates of 93.33 and 86.44% for P. expansum and A. ochraceus, respectively. The micelial growth inhibition was 28.45 and 21.0%, respectively. The antagonism showed by the selected yeasts could be used as a promising alternative tool to reduce and control the postharvest fungal spoilage of the fruits. However, further studies should be carried out in order to better elucidate the role of innocuous characters in antagonistic microorganisms, as well as the purification and characterization of new killer toxins |
| Disciplinas: | Biología |
| Palabras clave: | Microbiología, Levaduras asesinas, Biocontrol, Antibiosis, Actividad antifúngica, Kluyveromyces, Penicillium expansum, Aspergillus ochraceus |
| Keyword: | Biology, Microbiology, Killer yeasts, Biocontrol, Antibiosis, Antifungal activity, Kluyveromyces, Penicillium expansum, Aspergillus ochraceus |
| Texte intégral: | Texto completo (Ver HTML) |
