| Revue: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000374051 |
| ISSN: | 1516-8913 |
| Autores: | Demirel, Rasime1 Sariozlu, Nalan Yilmaz1 İlhan, Semra2 |
| Instituciones: | 1Anadolu University, Faculty of Science, Eskisehir. Turquía 2Eskisehir Osmangazi University, Faculty of Science and Art, Eskisehir. Turquía |
| Año: | 2013 |
| Periodo: | Nov-Dic |
| Volumen: | 56 |
| Número: | 6 |
| Paginación: | 980-984 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | In the present study, nine terverticillate Penicillium isolates (P. griseofulfum, P. puberulum, P. crustosum, P. aurantiogriseum, P. chrysogenum, P. primulinum, P. expansum, P. viridicatum, Eupenicillium egyptiacum) from 56 soil samples were characterized genetically by a PCR method. The DNAs of the strains were isolated using the glass beads and vortexing extraction method and then used for PCR amplification with the internal transcribed spacer 1 (ITS1) and ITS4 universal fungal specific primers. The ITS regions of fungal ribosomal DNA (rDNA) were sequenced through the CEQ 8000 Genetic Analysis System. ITS-5.8S sequences obtained were compared with those deposited in the GenBank Database. The results indicated that the identification of Penicillium species with PCR based methods provided significant information about the solution to taxonomy and improve food safety and to protect the users from harmful contaminants such as mycotoxins, which must be controlled during the production of agricultural materials as well as during the processing of food and feed |
| Disciplinas: | Biología |
| Palabras clave: | Hongos, Identificación molecular, Reacción en cadena de la polimerasa (PCR), Penicillium |
| Keyword: | Biology, Fungi, Molecular identification, Polymerase chain reaction (PCR), Penicillium |
| Texte intégral: | Texto completo (Ver HTML) |
