| Revue: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000392893 |
| ISSN: | 1516-8913 |
| Autores: | Ayaad, Tahany Hassan2 Al-Akeel, Rasha Khalifah1 Olayan, Ebtisam1 |
| Instituciones: | 1King Saud University, College of Science, Riyadh. Arabia Saudita 2Cairo University, College of Science, Giza. Egipto |
| Año: | 2015 |
| Periodo: | Nov-Dic |
| Volumen: | 58 |
| Número: | 6 |
| Paginación: | 905-912 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | The present investigation deals with the isolation and characterization of a lectin from Aedes aegypti (Ae aegypti) female mid gut extract that agglutinates various mammalian red blood cells (RBCs) human three groups A, B, and O (RH + ), mouse, rat, guinea - pig, sheep and goat erythrocytes. The highest activity of bot h crude and isolated mid gut lectins were detected against sheep RBCs. Using (NH 4 ) 2 SO 4 fractionation, ion - exchange and mannose - CNBr - Sepharose 6B affinity chromatography techniques , Ae. aegypti midgut lectin (Aelec) was purified to homogeneity. Isoelectric focusing (IEF) and reducing SDS/PAGE revealed that the isolated mid gut lectin had isoelectric point (PI) of 5.90, and subunits approximate molecular weights of 35.50 and 27.35 KDa. The hemagglutination (HA) of lectins were Ca 2+ - independent and heat - res istant. The sugar specificity of the purified Aelec was strongly inhibited by D (+) - mannose and raffinose, followed by D (+) glucose. N - acetyl - D - manosamine and N - acetyl - D - glucosamine were moderate inhibitors. None of the lectins were inhibited by the disac charides such as galactose, lactose, trehalose (IC50 up to 200 mM or fetuin up to 1%) but the glycosubstances mucin and laminarin were strong inhibitors up to very low concentrations (0.030 – 0.003%) |
| Disciplinas: | Biología, Química |
| Palabras clave: | Inmunología, Insectos, Bioquímica, Lectina, Purificación, Caracterización, Hemaglutinación, Aedes aegypti, Mosquitos |
| Keyword: | Biology, Chemistry, Immunology, Insects, Biochemistry, Lectin, Purification, Characterization, Hemagglutination, Aedes aegypti, Mosquitoes |
| Texte intégral: | Texto completo (Ver PDF) |
