Influence of starch and casein administered postruminally on small intestinal sodium-glucose cotransport activity and expression
Título del documento: Influence of starch and casein administered postruminally on small intestinal sodium-glucose cotransport activity and expression
Revue: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000331582
ISSN: 1516-8913
Autores: 1
2
2
3
3
1
Instituciones: 1Universidade Estadual de Maringa, Maringa, Parana. Brasil
2University of Kentucky, Lexington, Kentucky. Estados Unidos de América
3University of Guelph, Guelph, Ontario. Canadá
Año:
Periodo: Nov
Volumen: 50
Número: 6
Paginación: 963-970
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés Twenty-four Holstein steer calves (88 ± 3kg) with abomasal catheters were randomly assigned within blocks to one of four treatments. The treatments consisted of four abomasal infusions: water (control), 4 g/kg BW.d-1 of partially hydrolyzed starch (SH), 0.6 g/kg BW.d-1 of casein, and a mix of SH + casein. The small intestine was collected and five equidistan, 1m sites were identified (duodenum, jejunum 1, jejunum 2 jejunum 3 and ileum). Maltase specific activity in mucosal homogenate and brush border membrane vesicles, SGLT1 protein abundance, and sodium-dependent glucose uptake in brush border membrane vesicles did not differ between the calves receiving different abomasal infusion treatments. However, maltase specific activity in homogenates and brush border membrane vesicles increased four-fold from the duodenum to the first jejunal site before declining steadily towards the ileum (p=0.0145 p=0.0020, respectively). The SGLT1 abundance differed by intestinal sampling site (p=0.0162). These data indicated that cattle might not have the ability to alter the capacity for glucose uptake when challenged with different substrates and that the regulation of SGLT1 expression could differ between bovine and other species
Disciplinas: Medicina veterinaria y zootecnia,
Química,
Biología
Palabras clave: Nutrición animal,
Bioquímica,
Fisiología animal,
Ganado bovino,
Glucosa,
Maltasa,
Cotransportador sodio glucosa,
Almidón
Keyword: Veterinary medicine and animal husbandry,
Chemistry,
Biology,
Animal nutrition,
Biochemistry,
Animal physiology,
Cattle,
Glucose,
Maltase,
Sodium-glucose co-transporter,
Starch
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