| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000387747 |
| ISSN: | 1516-8913 |
| Autores: | Verma, Bala1 Kumar, Pradeep1 Karthik, Loganathan2 Dhanasekaran, Dharumadurai3 Babalola, Olubukola Oluranti4 Banakar, Shivakumar Pulakeshappa1 |
| Instituciones: | 1Shri JJT University, Department of Biosciences, Jhunjhunu, Rajasthan. India 2Shanghai Jiao Tong University, School of Life Sciences and Biotechnology, Shanghai. China 3Bharathidasan University, Department of Microbiology, Tiruchirapalli, Tamil Nadu. India 4North-West University, Faculty of Agriculture, Science and Technology, Mmabatho. Sudáfrica |
| Año: | 2015 |
| Periodo: | Jul-Ago |
| Volumen: | 58 |
| Número: | 4 |
| Paginación: | 628-635 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The present study was conducted for the isolation of potential bacteria from the desert soil, their molecular identification and prediction of restriction sites of the potential isolate using the bioinformatics tools. Production of the metabolites was done by inoculating in nutrient broth of pH 8.6. Metabolite was bluish-green in color; it was extracted and dried by using methanol and used for partial characterization by using GC-MS spectroscopy. Antibacterial activity was performed with the clinical human pathogenic isolates. The bacterium was identified as Pseudomonas sp. JJTBVK on the basis of 16S rRNA sequencing. The sequence was analyzed for the restriction cleavage sites, which showed that the sequence had various restriction sites for different enzymes. Antibacterial activity (MIC) of methanol extract of the bacterial culture broth showed antibacterial activity (MIC), which was 29, 30, 30 and 29 mm for Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Salmonella typhi, respectively. GC-MS analysis of the methanol extract showed the presence of naphth [2,3-B] azet-2 (1H) -one, 1-phenyl-, which was the characteristic compound showing the antibacterial activity |
| Disciplinas: | Biología, Química |
| Palabras clave: | Bacterias, Bioquímica, Pigmentos bacterianos, Cromatografía de gases, Espectrometría de masas, Actividad antibacteriana, Pseudomonas |
| Keyword: | Biology, Chemistry, Bacteria, Biochemistry, Bacterial pigments, Gas chromatography, Mass spectrometry, Antibacterial activity, Pseudomonas |
| Texto completo: | Texto completo (Ver HTML) |
