| Revue: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000365559 |
| ISSN: | 1516-8913 |
| Autores: | Carvalho, Dayse Cristina de1 Silva, Andre Luís Lopes da2 Schuck, Mariane Ruzza1 Purcino, Marivel1 Tanno, Guilherme Nakao1 Biasi, Luiz Antonio1 |
| Instituciones: | 1Universidade Federal do Parana, Departamento de Fitotecnia e Fitossanitarismo, Curitiba, Parana. Brasil 2Universidade Federal do Parana, Departamento de Engenharia Quimica, Curitiba, Parana. Brasil |
| Año: | 2013 |
| Periodo: | Mar-Abr |
| Volumen: | 56 |
| Número: | 2 |
| Paginación: | 191-202 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine was tested at 40 g.L-1. The BAP levels (1 and 5 μM) in solid and double-phase media were evaluated and compared with a control medium without BAP. Bordô had best in vitro growth than Chardonnay. Sucrose was a better carbohydrate source than glucose for both the cultivars. Bordô and Chardonnay had different amino acid preferences for some parameters. In conclusion, for in vitro shoot regeneration from the nodal segments, culture on solid medium with 5 μM BAP, 15 g.L-1 sucrose for Bordô and 45 g.L-1 sucrose for Chardonnay showed better results. Similarly, the supplementation of 40 g.L-1 arginine for Bordô and 40 g.L-1 arginine or glycine for Chardonnay showed better results |
| Disciplinas: | Biología, Agrociencias |
| Palabras clave: | Fisiología vegetal, Frutales, Propagación vegetativa, Medios de cultivo, Aminoácidos, Carbohidratos, Bencilaminopurina, Vitis labrusca, Vitis vinifera |
| Keyword: | Biology, Agricultural sciences, Plant physiology, Fruit trees, Vegetative propagation, Culture media, Amino acids, Carbohydrates, Benzylaminopurine, Vitis labrusca, Vitis vinifera |
| Texte intégral: | Texto completo (Ver HTML) |
