Comparison of High Performance Liquid Chromatography with Fluorescence Detector and with Tandem Mass Spectrometry methods for detection and quantification of Ochratoxin A in green and roasted coffee beans
Título del documento: Comparison of High Performance Liquid Chromatography with Fluorescence Detector and with Tandem Mass Spectrometry methods for detection and quantification of Ochratoxin A in green and roasted coffee beans
Revue: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000374021
ISSN: 1516-8913
Autores: 1
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Instituciones: 1Instituto Nacional de Metrologia, Qualidade e Tecnologia, Diretoria de Metrologia Cientifíca e Industrial, Duque de Caxias, Rio de Janeiro. Brasil
2Fundacao Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saude, Rio de Janeiro. Brasil
3Empresa Brasileira de Pesquisa Agropecuaria, Agroindustria de Alimentos, Guaratiba, Rio de Janeiro. Brasil
Año:
Periodo: Nov-Dic
Volumen: 56
Número: 6
Paginación: 911-920
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Two analytical methods for the determination and confirmation of ochratoxin A (OTA) in green and roasted coffee samples were compared. Sample extraction and clean-up were based on liquid–liquid phase extraction and immunoaffinity column. The detection of OTA was carried out with the high performance liquid chromatography (HPLC) combined either with fluorescence detection (FLD), or positive electrospray ionization (ESI+) coupled with tandem mass spectrometry (MS–MS). The results obtained with the LC-ESI-MS/MS were specific and more sensitive, with the advantages in terms of unambiguous analyte identification, when compared with the HPLC-FLD
Disciplinas: Química,
Agrociencias
Palabras clave: Química de alimentos,
Análisis químico,
Ocratoxina A,
Cromatografía líquida de alta resolución (HPLC),
Espectrometría de masas,
Café tostado,
Café
Keyword: Chemistry,
Agricultural sciences,
Food chemistry,
Chemical analysis,
Ochratoxin A,
High performance liquid chromatography (HPLC),
Mass spectrometry,
Roasted coffee,
Coffee
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