Revue: | Brazilian archives of biology and technology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000374018 |
ISSN: | 1516-8913 |
Autores: | Gu, Lijiang1 Zhang, Han1 Guo, Huiming1 Cheng, Hongmei1 |
Instituciones: | 1Chinese Academy of Agricultural Sciences, Biotechnology Research Institute, Beijing. China |
Año: | 2013 |
Periodo: | Nov-Dic |
Volumen: | 56 |
Número: | 6 |
Paginación: | 895-903 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental |
Resumen en inglés | C-repeat-binding factors (CBFs) are a type of important regulon in stress-related signal transduction pathways that control plant tolerance of abiotic stress. Ammopiptanthus mongolicus is the only evergreen broadleaf shrub in the northwest desert of China. The species shows strong resistance to environmental stress, especially to cold stress. An A. mongolicus CBF1 gene (AmCBF1) was cloned and transformed into tobacco. Expression of AmCBF1 could be detected in A. mongolicus shortly after exposure to low temperature of 4°C. Analysis on ratio of electrolytic leakage, soluble sugar content, free proline content, malondialdehyde (MDA) content and peroxidase (POD) activity before and after cold treatment (4°C) for 24 h indicated AmCBF1 conferred higher cold tolerance to AmCBF1 transgenic tobacco compared with the wild type and empty vector transformed tobacco |
Disciplinas: | Biología |
Palabras clave: | Angiospermas, Fisiología vegetal, Genética, Clonación, Plantas transgénicas, Resistencia, Congelación, Ammopiptanthus mongolicus, Tabaco |
Keyword: | Biology, Angiosperms, Genetics, Plant physiology, Cloning, Transgenic plants, Resistance, Freezing, Ammopiptanthus mongolicus, Tobacco |
Texte intégral: | Texto completo (Ver HTML) |