| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000366264 |
| ISSN: | 1516-8913 |
| Autores: | Anschau, Andreia1 Santos, Lucielen Oliveira dos2 Alegre, Ranulfo Monte1 |
| Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos, Campinas, Sao Paulo. Brasil 2Universidade Federal do Rio Grande, Escola de Quimica e Alimentos, Rio Grande, Rio Grande do Sul. Brasil |
| Año: | 2013 |
| Periodo: | Sep-Oct |
| Volumen: | 56 |
| Número: | 5 |
| Paginación: | 849-857 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bioreactor resulted 241.3 mg/L GSH after 96 h in batch fermentation without amino acids addition and the concentration of biomass was 12.1 g/L. In batch fermentation with the addition of the three amino acids (4 mM cysteine, glycine and glutamic acid at 32 h), biomass reached to 25 g/L and GSH, 236.1 mg/L at 96 h of fermentation. The strategy of precursor amino acids addition is a key aspect in increasing the synthesis of GSH |
| Disciplinas: | Química |
| Palabras clave: | Fermentaciones, Química de alimentos, Caña de azúcar, Melaza, Saccharomyces cerevisiae, Glutatión, Producción, Glicerol, Aminoácidos, Metodología de superficie de respuesta |
| Keyword: | Chemistry, Fermentation, Food chemistry, Sugar cane, Molasses, Saccharomyces cerevisiae, Glutathione, Production, Glycerol, Amino acids, Response surface methodology |
| Texto completo: | Texto completo (Ver HTML) |
