Demonstration by RNA interference of a new molecular mechanism for resistance to an oomycete in tobacco plants
Título del documento: Demonstration by RNA interference of a new molecular mechanism for resistance to an oomycete in tobacco plants
Revue: Biotecnología aplicada
Base de datos: PERIÓDICA
Número de sistema: 000351141
ISSN: 0864-4551
Autores: 1
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2
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Instituciones: 1Centro de Ingeniería Genética y Biotecnología, División de Plantas, La Habana. Cuba
2Instituto de Investigaciones del Tabaco, La Habana. Cuba
Año:
Volumen: 27
Número: 3
Paginación: 242-244
País: Cuba
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Oomycetes constitute an important agricultural pathogen for plants of the Solanaceae family. Black shank, caused by Phytophthora parasitica var. nicotianae, is one of the most important diseases affecting tobacco plantations in Cuba and worldwide, constituting an occasional cause of major economic losses. We have constructed a subtractive library with the aim of identifying tobacco genes involved in the resistance to this oomycete. The analysis by realtime PCR confirmed, for the first time, the patterns of differential expression between resistance and susceptibility reactions for the identified genes. This constitutes the first report demonstrating the pattern of expression of the glutathione-S-transferase (GST) gene in varieties and species independently of the degree of resistance or susceptibility to the pathogen. The gene was cloned in a plant transformation vector for gene silencing, and transgenic N. tabacum plants with silenced GST were obtained. They significantly displayed increased resistance to the disease, in highly susceptible plants, in comparison to control plants. This work constitutes the first report on the role of GST as a negative regulator of the defensive response to oomycetes, as well as the first instance where RNA interference technology has ever been used to obtain resistance to diseases produced by Oomycetes
Disciplinas: Agrociencias
Palabras clave: Fitopatología,
Hongos fitopatógenos,
Tabaco,
Resistencia a plagas,
Biología molecular,
Nicotiana tabacum,
Phytophthora parasitica,
Oomycetes
Keyword: Agricultural sciences,
Phytopathology,
Phytopathogenic fungi,
Tobacco,
Pests resistance,
Molecular biology,
Nicotiana tabacum,
Phytophthora parasitica,
Oomycetes
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