| Revista: | Biotecnología aplicada |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000353533 |
| ISSN: | 0864-4551 |
| Autores: | Falcón, Alejandro1 Rodríguez, Aída T2 Ramírez, Miguel A2 Rivero, Deyanira2 Martínez, Benedicto3 Cabrera, Juan C4 Costales, Daimy1 Cruz, Ariel2 González, Luis G5 |
| Instituciones: | 1Instituto Nacional de Ciencias Agrícolas, Departamento de Fisiología y Bioquímica Vegetal, La Habana. Cuba 2Instituto Nacional de Ciencias Agrícolas, Estación experimental del Arroz, Los Palacios, Pinar del Río. Cuba 3Centro Nacional de Sanidad Agropecuaria, Departamento de Protección de Plantas, La Habana. Cuba 4Universite de Namour, Faculte de Agronomie, Namur. Bélgica 5Universidad de Granma, Facultad de Agronomía, Granma. Cuba |
| Año: | 2010 |
| Volumen: | 27 |
| Número: | 4 |
| Paginación: | 305-309 |
| País: | Cuba |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Studies were carried out as part of the Agriculture Biotechnology program, to prepare and characterize chemically and biologically different chitosans obtained from Cuban lobster chitin. Chitosan polymers were subjected to acid and enzymatic hydrolysis by using low-cost commercial enzymatic preparations, and the resulting oligosaccharide mixtures were further characterized. Their potential antimicrobial activities were also evaluated versus fungi and oomycetes, also testing their ability to induce defensive and protective responses in tobacco and rice plants against two economically relevant pests, Phytophthora nicotianae and Pyricularia grisea, respectively. With the aid of international collaboration, different oligochitosans mixtures were compared for activating defensive responses in suspension cultures of Arabidopsis thaliana cells. These results bring knowledge on the physical-chemical properties of the chitosans obtained, such as molar mass and acetylation grade, and their influence on activating defensive responses, the inhibition of growth in pathogens and the induction of resistance in tobacco and rice plants. Some of these chitosan derivatives were selected as possible active components to protect both type of cultivars, being applied at field-scale to evaluate their effects for the main natural pathogens and bringing very promising results. This research allowed us to establish a methodology for preparing oligochitosans, and results shown inhere were part of BSc, MSc and PhD theses, and were also published in more than 20 scientific papers and presented in more than 40 scientific conferences |
| Disciplinas: | Agrociencias, Biología |
| Palabras clave: | Fisiología vegetal, Biotecnología, Bioquímica, Quitosana, Actividad antifúngica, Resistencia inducida |
| Keyword: | Agricultural sciences, Biology, Plant physiology, Biotechnology, Biochemistry, Chitosan, Antifungal activity, Induced resistance |
| Texto completo: | Texto completo (Ver PDF) |
