Modulation of IL-10/IL-10R expression by mafosfamide, a derivative of 4-hydroxycyclophosphamide, in a rat B-cell lymphoma
Título del documento: Modulation of IL-10/IL-10R expression by mafosfamide, a derivative of 4-hydroxycyclophosphamide, in a rat B-cell lymphoma
Revista: Biocell
Base de datos: PERIÓDICA
Número de sistema: 000352023
ISSN: 0327-9545
Autores: 1
1
1
Instituciones: 1Universidad Nacional de Rosario, Instituto de Genética Experimental, Rosario, Santa Fe. Argentina
Año:
Volumen: 36
Número: 2
Paginación: 91-95
País: Argentina
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés We have already shown that IL-10 plays an important role in immunosuppression and metastatic dissemination in the rat B-cell lymphoma L-TACB model. It was suggested that the up-regulation of IL-10 production and IL-10 receptor (IL-10R) expression would be part of the transition from primary tumor to metastatic phenotype and that IL-10, besides its immunosuppressive activity, may act as a growth factor for metastatic L-TACB cells. The treatment of L-TACB-bearing rats with a single low-dose cyclophosphamide decreased IL-10 production, reverted immunosuppression and induced the immunologic rejection of tumor metastasis without any effect on primary tumor growth. Our current aim was to investigate the effects of cyclophosphamide on the expression of IL-10 and IL-10R on primary and metastatic L-TACB cells. Considering that cyclophosphamide is a prodrug, we used mafosfamide, a compound that yields in vitro the same active metabolites as cyclophosphamide does in vivo. Mafosfamide induced down-regulation of IL-10 production and IL-10R expression on metastatic cells and, concomitantly, inhibited metastatic cell proliferation. We suggest that mafosfamide would inhibit the regulatory loop mediated by the IL-10/IL-10R system and, as a consequence, metastatic cell proliferation. These results may have a considerable impact on the design of new therapies for metastatic lymphomas
Disciplinas: Biología,
Medicina
Palabras clave: Medicina experimental,
Oncología,
Interleucina 10,
Metástasis,
Ciclofosfamida,
Linfoma de células B,
Proliferación celular
Keyword: Biology,
Medicine,
Experimental medicine,
Oncology,
Interleukin 10,
Metastasis,
Cyclophosphamide,
B cells lymphoma,
Cell proliferation
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