| Revue: | Biocell |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000366025 |
| ISSN: | 0327-9545 |
| Autores: | Varecha, Miroslav1 Paclova, Daniela2 Prochazkova, Jirina2 Matula, Pavel1 Cmarko, Dusan3 Kozubek, Michal1 |
| Instituciones: | 1Masaryk University, Faculty of Informatics, Brno, Jihomoravsky. República Checa 2Masaryk University, Faculty of Science, Brno, Jihomoravsky. República Checa 3Charles University, Faculty of Medicine, Praga. República Checa |
| Año: | 2012 |
| Periodo: | Dic |
| Volumen: | 36 |
| Número: | 3 |
| Paginación: | 121-126 |
| País: | Argentina |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Our results show that tumoral telomerase-positive (TP) AIF knockdown cell lines display significant increase in superoxide production in comparison to control cells, while a non-tumoral cell line and tumoral telomerase-negative cell lines with alternative lengthening of telomeres (ALT) show a decrease in superoxide production. According to these results, we can conclude that AIF knockdown disrupts function of complex I and therefore increases the superoxide production in mitochondria. The distinct effect of AIF depletion in various cell lines could result from recently discovered activity of telomerase in mitochondria of TP cancer cells, but this hypothesis needs further investigation |
| Disciplinas: | Biología |
| Palabras clave: | Biología celular, Apoptosis, Respiración, Superóxido, Microscopía de fluorescencia, Mitocondrias, Telomerasa |
| Keyword: | Biology, Cell biology, Apoptosis, Respiration, Superoxide, Fluorescence microscopy, Mitochondria, Telomerase |
| Texte intégral: | Texto completo (Ver PDF) |
