In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line
Título del documento: In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line
Revue: Biocell
Base de datos: PERIÓDICA
Número de sistema: 000330405
ISSN: 0327-9545
Autores: 1
1
Instituciones: 1University of Pretoria, Department of Physiology, Pretoria, Transvaal. Sudáfrica
Año:
Periodo: Ago
Volumen: 34
Número: 2
Paginación: 71-79
País: Argentina
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM’s in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4μM. In addition, mitotic indices revealed that 2ME-BM induces a G2M block. The latter was confirmed by flow cytometric analyses where increased sub-G1 and G2/M fractions, as well as an increase in cyclin B1 levels were observed. Further in vitro research into the mechanism of this potentially useful anticancer compound is thus warranted
Disciplinas: Biología,
Medicina
Palabras clave: Biología celular,
Oncología,
Bioensayos,
2-metoxiestradiol-bis-sulfamato,
Crecimiento celular,
Ciclo celular,
Adenocarcinoma,
Apoptosis,
Autofagia
Keyword: Biology,
Medicine,
Cell biology,
Oncology,
Bioassays,
2-methoxyestradiol-bis-sulphamate,
Cell growth,
Cell cycle,
Adenocarcinoma,
Apoptosis,
Autophagy
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