Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
Título del documento: Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
Revue: Biocell
Base de datos: PERIÓDICA
Número de sistema: 000335532
ISSN: 0327-9545
Autores: 1
1
1
Instituciones: 1Universidad de Buenos Aires, Facultad de Agronomía, Buenos Aires. Argentina
Año:
Volumen: 35
Número: 1
Paginación: 1-7
País: Argentina
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77%) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. Developmental rates did not differ between diploidization strategies, even when three agents were employed to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor
Disciplinas: Biología
Palabras clave: Biología celular,
Embriones partenogenéticos,
Meiosis,
Microfilamentos,
Citocalasina B,
Cuerpos polares
Keyword: Biology,
Cell biology,
Parthenogenetic embryos,
Meiosis,
Microfilaments,
Cytochalasin B,
Polar bodies
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