A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR
Título del documento: A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR
Revista: Biocell
Base de datos: PERIÓDICA
Número de sistema: 000335536
ISSN: 0327-9545
Autores: 1
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Instituciones: 1Universidade de Ribeirao Preto, Unidade de Biotecnologia, Ribeirao Preto, Sao Paulo. Brasil
Año:
Volumen: 35
Número: 1
Paginación: 35-36
País: Argentina
Idioma: Inglés
Tipo de documento: Nota breve o noticia
Enfoque: Experimental, aplicado
Resumen en inglés E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR
Disciplinas: Biología
Palabras clave: Bacterias,
Microbiología,
Aislamiento bacteriano,
PCR,
Ehrlichia canis
Keyword: Biology,
Bacteria,
Microbiology,
Bacterial isolation,
PCR,
Ehrlichia canis
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