Avaliação da urina e de leucócitos como amostras biológicas para a detecção ante mortem do vírus da cinomose canina por RT-PCR em cães naturalmente infectados



Título del documento: Avaliação da urina e de leucócitos como amostras biológicas para a detecção ante mortem do vírus da cinomose canina por RT-PCR em cães naturalmente infectados
Revue: Arquivo brasileiro de medicina veterinaria e zootecnia
Base de datos: PERIÓDICA
Número de sistema: 000287493
ISSN: 0102-0935
Autores: 1
1
2
Instituciones: 1Universidade Estadual de Londrina, Laboratorio de Virología Animal,
2Universidade Estadual de Londrina, Laboratorio de Virología Animal, Londrina, Parana. Brasil
Año:
Periodo: Feb
Volumen: 59
Número: 1
Paginación: 253-257
País: Brasil
Idioma: Portugués
Tipo de documento: Nota breve o noticia
Enfoque: Experimental, analítico
Resumen en inglés Urine and leucocytes were comparatively evaluated as clinical samples for ante mortem detection of the canine distemper virus (CDV) by a reverse transcription-polymerase chain reaction (RT-PCR) assay. One hundred and eighty eight dogs with clinical symptoms of distemper, were distributed in three groups. The group A was constituted of 93 dogs with systemic signs of distemper; the group B by 11 dogs with neurological signs, and the group C by 84 dogs that presented simultaneously systemic and neurological signs. In 66.5% (125/188) of the dogs was amplified an amplicon with 287 base pair of the CDV nucleoprotein gene. In 60.8% (76/125) of the animals the CDV was detected simultaneously in the urine and leucocytes, and in 39.2% (49/125) of the dogs just a type of clinical sample (urine: n=37; leucocytes: n=12) was positive. These results demonstrate that the different forms of clinical distemper disease can hinder the choice of only one type of clinical sample to carry out the ante mortem etiological diagnosis of CDV infection, and false-negative results can be generated
Disciplinas: Medicina veterinaria y zootecnia,
Biología
Palabras clave: Pequeñas especies,
Virus,
Perros,
Moquillo,
Diagnóstico,
RT-PCR
Keyword: Veterinary medicine and animal husbandry,
Biology,
Companion animals,
Virus,
Dogs,
Distemper,
Diagnosis,
RT-PCR
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