| Revue: | Annals of hepatology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000409087 |
| ISSN: | 1665-2681 |
| Autores: | Ma, Hucheng1 Shi, Xiaolei1 Yuan, Xianwen1 Ding, Yitao1 |
| Instituciones: | 1Nanjing University, Medical School, Nanjing, Jiangsu. China |
| Año: | 2016 |
| Volumen: | 15 |
| Número: | 2 |
| Paginación: | 260-270 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Aplicado, descriptivo |
| Resumen en inglés | Uncontrolled hapatic inflammatory response is regarded as the primary pathological mechanism of acute liver failure and impairs the regeneration of hepatocytes and stem cell grafts. Interleukin-1 plays a key role for activating immune and inflammatory response. Recently, siRNA has made quite a few progresses in treating inflammatory response. To assess the effect of IL-1β siRNA adenovirus on MSC and the therapeutic effect of MSC combined with IL-1β siRNA adenovirus in ALF. We implanted MSC or/and IL-1β siRNA adenovirus via the tail vein, using CCl4-induced ALF in a mice model. Mice were sacrificed at different time points. Blood samples and liver tissues were collected. Hepatic injury, liver regeneration, cytokines (CXCL1, IL-1β, IL-10, IL-6, VEGF and HGF), animal survival and vital MSC were assessed after cell transplantation. Results. Results. MSC combined with IL-1β siRNA reduced the inflammatory levels and prevented liver failure. These animals administrated with MSC and IL-1β siRNA also exhibited improved liver regeneration and increased survival rates. Immunohistochemistry and fluorescence microscopy revealed the number of vital MSC in ALF + MSC + IL-1β siRNA group were significantly more than that in ALF + MSC group. IL-1β siRNA adenovirus could enhance MSC ability of tissue regeneration through increasing its survival rate. Accordingly, combination of IL-1β siRNA adenovirus and MSC had a synergistic effect on acute liver failure |
| Disciplinas: | Medicina |
| Palabras clave: | Gastroenterología, Medicina experimental, Falla hepática aguda, Interleucina 1 beta, Regeneración tisular, ARN de interferencia |
| Keyword: | Medicine, Experimental medicine, Gastroenterology, Acute liver failure, Interleukin-1beta, Tissue regeneration, Interference RNA |
| Texte intégral: | Texto completo (Ver PDF) |
