| Revue: | Annals of hepatology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000413427 |
| ISSN: | 1665-2681 |
| Autores: | Longo, Larisse1 Sinigaglia-Fratta, Leila Xavier1 Weber, Giovana R1 Janz-Moreira, Andrea1 Kretzmann, Nelson A1 Grezzana-Filho, Tomaz de J.M1 Possa-Marroni, Norma1 Corso, Carlos O1 Schmidt-Cerski, Carlos T1 Reverbel da Silveira, Themis1 Alvares da Silva, Mario R1 Santos, Jorge L. dos1 |
| Instituciones: | 1Universidade Federal do Rio Grande do Sul, Hospital de Clinicas de Porto Alegre, Porto Alegre, Rio Grande do Sul. Brasil |
| Año: | 2016 |
| Periodo: | Ene-Feb |
| Volumen: | 15 |
| Número: | 1 |
| Paginación: | 101-120 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Topical hypothermia (TH) and ischemic preconditioning (IPC) are used to decrease I/R injury. The efficacy of isolated or combined use of TH and IPC in the liver regarding inflammation and cytoprotection in early ischemia/reperfusion (I/R) injury needs to be evaluated. Material and methods. Material and methods. Wistar rats underwent 70% liver ischemia for 90 min followed by 120 min of reperfusion. Livers of animals allocated in the sham, normothermic ischemia (NI), IPC, TH, and TH+IPC groups were collected for molecular analyses by ELISA and Western blot, aiming to compare proinflammatory, anti-inflammatory, and antioxidant profiles. Results. Compared with NI, TH presented decreased tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-12 concentrations and increased IL-10 levels. TH animals displayed lower inducible nitric oxide synthase (iNOS) and higher endothelial nitric oxide synthase (eNOS) expressions. NAD(P)H-quinone oxidoreductase-1(NQO1) expression was also lower with TH. Isolated IPC and NI were similar regarding all these markers. TH+IPC was associated with decreased IL-12 concentration and reduced iNOS and NQO1 expressions, similarly to isolated TH. Expression of Kelch-like ECH-associated protein (Keap)-1 was increased and expression of nuclear and cytosolic nuclear erythroid 2-related factor 2 (Nrf2) was decreased with TH+IPC vs. NI. Conclusion. Conclusion. TH Conclusion. was the most effective method of protection against early I/R injury. Isolated IPC entailed triggering of second-line antioxidant defense enzymes. Combined TH+IPC seemed to confer no additional advantage over isolated TH in relation to the inflammatory process, but had the advantage of completely avoid second-line antioxidant defense enzymes |
| Disciplinas: | Medicina |
| Palabras clave: | Gastroenterología, Medicina experimental, Lesiones hepáticas, Fisiopatología, Inflamación, Hipotermia, Antioxidantes |
| Keyword: | Medicine, Experimental medicine, Gastroenterology, Liver injuries, Physiopathology, Inflammation, Hypothermia, Antioxidants |
| Texte intégral: | Texto completo (Ver PDF) |
