| Revue: | Annals of hepatology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000415014 |
| ISSN: | 1665-2681 |
| Autores: | Pizarro, María Dolores1 Mediavilla, María Gabriela1 Berardi, Florencia1 Tiribelli, Claudio2 Rodríguez, Joaquín V1 Mamprin, María Eugenia3 |
| Instituciones: | 1Universidad Nacional de Rosario, Centro Binacional de Criobiología Clínica y Aplicada, Rosario, Santa Fe. Argentina 2Centro Studi Fegato, Trieste, Friuli Venezia Giuli. Italia 3Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas, Rosario, Santa Fe. Argentina |
| Año: | 2014 |
| Periodo: | Mar-Abr |
| Volumen: | 13 |
| Número: | 2 |
| Paginación: | 256-264 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | This work focuses on ammonia metabolism of Liver Microorgans (LMOs) after cold preservation in a normothermic reoxygenation system (NRS). We have previously reported the development of a novel preservation solution, Bes-Gluconate-PEG 35 kDa (BG35) that showed the same efficacy as ViaSpan® to protect LMOs against cold preservation injury. The objective of this work was to study mRNA levels and activities of two key Urea Cycle enzymes, Carbamyl Phosphate Synthetase I (CPSI) and Ornithine Transcarbamylase (OTC), after preservation of LMOs in BG35 and ViaSpan® and the ability of these tissue slices to detoxify an ammonia overload in a NRS model. Material and methods. After 48 h of cold storage (0°C in BG35 or ViaSpan®) LMOs were rewarmed in KHR containing an ammonium chloride overload (1 mM). We determined ammonium detoxification capacity (ADC), urea synthesis and enzyme activities and relative mRNA levels for CPSI and OTC. Results. At the end of reoxygenation LMOs cold preserved in BG35 have ADC and urea synthesis similar to controls. ViaSpan® group demonstrated a lower capacity to detoxify ammonia and to synthesize urea than fresh LMOs during the whole reoxygenation period which correlated with the lower mRNA levels and activities for CPSI and OTC observed for this group. Conclusion. We demonstrate that our preservation conditions (48 hours, BG35 solution, anoxia, 0ºC) did not affect ammonia metabolism of cold preserved LMOs maintaining the physiological and biochemical liver functions tested, which allows their future use as biological component of a BAL system |
| Disciplinas: | Medicina |
| Palabras clave: | Gastroenterología, Microbiología, Hígado, Criopreservación, Microbiota hepática, Trasplante de hígado, Reoxigenación |
| Keyword: | Medicine, Gastroenterology, Microbiology, Liver, Cryopreservation, Liver microbiota, Liver transplantation, Reoxygenation |
| Texte intégral: | Texto completo (Ver PDF) |
