| Revue: | Anais da Academia Brasileira de Ciencias |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000424403 |
| ISSN: | 0001-3765 |
| Autores: | Abreu, Dilayla K. de1 Monteiro, Janaina M2 Souza, Carolina C1 Karam, Rafael G1 Fernandes, Renata A1 Lessa, Thais B1 Fagliari, Jose J3 Miglino, Maria Angelica1 Ambrosio, Carlos Eduardo4 |
| Instituciones: | 1Universidade de Sao Paulo, Faculdade de Medicina Veterinaria e Zootecnia, Sao Paulo. Brasil 2Hospital Israelita Albert Einstein, Centro de Pesquisa Experimental, Sao Paulo. Brasil 3Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias Agrarias e Veterinarias, Jaboticabal, Sao Paulo. Brasil 4Universidade de Sao Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Pirassununga, Sao Paulo. Brasil |
| Año: | 2018 |
| Periodo: | Sep |
| Volumen: | 90 |
| Número: | 3 |
| Paginación: | 2977-2990 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Duchenne Muscular Dystrophy (DMD) is the most common X-linked muscular disease affecting humans. The Golden Retriever Muscular Dystrophy model (GRMD) is considerthe most suitable for several studies. This assay aims to quantify lymphocyte subpopulations CD4, CD5, and CD8, and standardize, the serum electrophoretic profile, to understand their contribution to the pathologic process in normal Golden Retriever dogs (GR group) and dystrophic´s (GRMD group), through the umbilical cord blood, in dogs aged from 2 to 3 months (GR II and GRMD II), and in dogs over 1 year of age (GR III and GRMD III). No significant differences were observed between the CD8+ lymphocyte subpopulations of the groups studied. The CD4+ and CD5+ lymphocyte subpopulations were significantly higher in the GRMD III group compared to the GR III group. Twenty-two different proteins in the gel were identified. The serum concentrations of the proteins belonging to the GR I and GRMD I groups were significantly lower than those of the other groups. We show that expression of acute phase proteins are worst during the aging of the dogs. We hope to expand knowledge to better understand the GRMD model and the translational data |
| Disciplinas: | Medicina |
| Palabras clave: | Inmunología, Neurología, Medicina experimental, Distrofia muscular de Duchenne, Modelos animales, Fenotipificación inmunológica, Proteínas séricas |
| Keyword: | Immunology, Neurology, Experimental medicine, Duchenne muscular dystrophy, Animal models, Immunophenotyping, Serum proteins |
| Texte intégral: | Texto completo (Ver HTML) |
