| Revue: | Anais da Academia Brasileira de Ciencias |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000400488 |
| ISSN: | 0001-3765 |
| Autores: | Fonseca, Leonardo M1 Garcez, Tatiana C1 Penha, Luciana1 Lima, Leonardo Freire de1 Maes, Emmanuel2 Costa, Kelli M1 Mendonca-Previato, Lucia1 Previato, Jose O1 |
| Instituciones: | 1Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Rio de Janeiro. Brasil 2Universite des Sciences et Technologies de Lille, Unite de Glycobiologie Structurale et Fonctionnelle, Lille, Cotes du Nord. Francia |
| Año: | 2016 |
| Periodo: | Sep |
| Volumen: | 88 |
| Número: | 3 |
| Paginación: | 1519-1530 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | One of the main obstacles to the treatment of Chagas disease is the genetic and phenotypical variance displayed by T. cruzi strains, resulting in differences in morphology, virulence, pathogenicity and drug susceptibility. To better understand the role of glycoconjungates in Chagas disease, we performed the molecular characterization of the O-linked chains from mucins and glycoinositolphospholipids (GIPLs) of the Silvio X10 clone 1 strain. We demonstrated the presence of a β-galactofuranose (β-Galf) unity linked to the O-4 position of the α-N-acetylglucosamine (α-GlcNAc)O-4 in Tc-mucins. GIPLs analysis showed that the lipidic portion is exclusively composed of ceramide and the PI-oligossacharidic portion contains the Man4(AEP)GlcN-Ins-PO4 core, substituted by ethanolamine-phosphate (EtNP) on the third distal mannose from inositol, which may or may not have a terminal β Galf unity. These results confirm the classification of the Silvio X10/1 strain in group T. cruzi I. Again, it is noted that the study of T. cruzi surface glycoconjugates confirm the molecular results and the hypothesis that surface glycoconjugates may be interesting biomarker for the differentiation of trypanosomatid strains |
| Disciplinas: | Biología |
| Palabras clave: | Protozoarios, Bioquímica, Glucanos, Mucinas, Glucoinositolfosfolípidos, Trypanosoma cruzi |
| Keyword: | Biology, Protozoa, Protozoans, Biochemistry, Glycans, Mucins, Glycoinositolphospholipids, Trypanosoma cruzi |
| Texte intégral: | Texto completo (Ver HTML) |
