Antiproliferative activity in tumor cell lines, antioxidant capacity and total phenolic, flavonoid and tannin contents of Myrciaria floribunda
Título del documento: Antiproliferative activity in tumor cell lines, antioxidant capacity and total phenolic, flavonoid and tannin contents of Myrciaria floribunda
Revue: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000409015
ISSN: 0001-3765
Autores: 1
2
1
1
1
1
1
3
4
1
Instituciones: 1Universidade Federal Fluminense, Faculdade de Farmacia, Niteroi, Rio de Janeiro. Brasil
2Universidade Federal do Rio de Janeiro, Centro de Ciencias da Saude, Rio de Janeiro. Brasil
3Universidade do Estado do Rio de Janeiro, Faculdade de Formacao de Professores, Sao Goncalo, Rio de Janeiro. Brasil
4Universidade Estadual de Campinas, Centro Pluridisciplinar de Pesquisas Quimicas, Biologicas e Agricolas, Campinas, Sao Paulo. Brasil
Año:
Periodo: Jun
Volumen: 89
Número: 2
Paginación: 1111-1120
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is a native plant species of the Atlantic Rain Forest, from north to south of Brazil. The lyophilized ethyl acetate extract from the leaves of M. floribunda was investigated for its antiproliferative activity in tumor cell lines, antioxidant capacity and its total phenolic, flavonoid and tannin contents. Antiproliferative activity was tested in vitro against seven human cancer cells and against immortalized human skin keratinocytes line (HaCat, no cancer cell). Antioxidant activity was determined using 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbing capacity (ORAC) assays and total phenolic, flavonoid and tannin contents were determined by spectrophotometric techniques. Ethyl acetate extract of M. floribunda exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 69.70 and 172.10 µg/mL. For HaCat cell, TGI value was 213.60 µg/mL. M. floribunda showed a strong antioxidant potential: EC50 of 45.89±0.42 µg/mL and 0.55±0.05 mmol TE/g for DPPH and ORAC, respectively. Total phenolic content was 0.23±0.013g gallic acid equivalents (GAE)/g extract and exhibited 13.10±1.60% of tannins content. The content of flavonoid was 24.08±0.44% expressed as rutin equivalents. These results provide a direction for further researches about the antitumoral potential of M. floribunda
Disciplinas: Biología,
Química
Palabras clave: Angiospermas,
Fitoquímica,
Química farmacéutica,
Flavonoides,
Taninos,
Capacidad antioxidante,
Actividad antiproliferativa,
Myrciaria floribunda,
Myrtaceae
Keyword: Biology,
Chemistry,
Angiosperms,
Medicinal chemistry,
Phytochemistry,
Flavonoids,
Tannins,
Antioxidant capacity,
Antiproliferative activity,
Myrciaria floribunda,
Myrtaceae
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