| Revue: | Anais da Academia Brasileira de Ciencias |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000412271 |
| ISSN: | 0001-3765 |
| Autores: | Aguiar, Raquel R. de1 Vale, Daniela F1 Silva, Renato M. da1 Muniz, Yolanda P1 Antunes, Fernanda1 Logullo, Carlos1 Oliveira, Andre L.A1 Almeida, Adriana J. de1 |
| Instituciones: | 1Universidade Estadual do Norte Fluminense Darcy Ribeiro, Unidade de Experimentacao Animal, Campos dos Goytacazes, Rio de Janeiro. Brasil |
| Año: | 2017 |
| Periodo: | Sep |
| Volumen: | 89 |
| Número: | 3 |
| Paginación: | 1683-1690 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Ischemia is responsible for many metabolic abnormalities in the heart, causing changes in organ function. One of modifications occurring in the ischemic cell is changing from aerobic to anaerobic metabolism. This change causes the predominance of the use of carbohydrates as an energy substrate instead of lipids. In this case, the glycogen is essential to the maintenance of heart energy intake, being an important reserve to resist the stress caused by hypoxia, using glycolysis and lactic acid fermentation. In order to study the glucose anaerobic pathways utilization and understand the metabolic adaptations, New Zealand white rabbits were subjected to ischemia caused by Inflow occlusion technique. The animals were monitored during surgery by pH and lactate levels. Transcription analysis of the pyruvate kinase, lactate dehydrogenase and phosphoenolpyruvate carboxykinase enzymes were performed by qRT-PCR, and glycogen quantification was determined enzymatically. Pyruvate kinase transcription increased during ischemia, followed by glycogen consumption content. The gluconeogenesis increased in control and ischemia moments, suggesting a relationship between gluconeogenesis and glycogen metabolism. This result shows the significant contribution of these substrates in the organ energy supply and demonstrates the capacity of the heart to adapt the metabolism after this injury, sustaining the homeostasis during short-term myocardial ischemia |
| Disciplinas: | Medicina |
| Palabras clave: | Medicina experimental, Sistema cardiovascular, Conejos, Isquemia miocárdica, Gluconeogénesis |
| Keyword: | Medicine, Cardiovascular system, Experimental medicine, Rabbits, Myocardial ischemia, Gluconeogenesis |
| Texte intégral: | Texto completo (Ver HTML) |
