| Revista: | Acta scientiarum. Biological sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000433588 |
| ISSN: | 1679-9283 |
| Autores: | Rossato, Marieli1 Schumacher, Pedro Vitor1 Costa-Netto, Antônio Paulino da2 Stein, Vanessa Cristina3 Reis, Edésio Fialho dos2 Vilela, Marlúcia Souza Pádua4 Paiva, Luciano Vilela4 |
| Instituciones: | 1Universidade Federal de Lavras, Programa de Pos-Graduacao em Fisiologia Vegetal, Lavras, Minas Gerais. Brasil 2Universidade Federal de Jatai, Jatai, Goias. Brasil 3Universidade de Sao Joao Del Rei, Divinopolis, Minas Gerais. Brasil 4Universidade Federal de Lavras, Lavras, Minas Gerais. Brasil |
| Año: | 2019 |
| Volumen: | 41 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Campomanesia adamantiumis a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA, NAA or picloram. Cytochemical and SEM analyses revealed cellular characteristics of the formation of meristematic centers that indicated 4.14 μM of picloram to be the best treatment for induction of embryogenic calli, and demonstrating their embryogenic potential. The treatment was used to establish a callus growth curve, from which it was inferred that calli should be transferred to new culture media on the 28thday to maintain cell viability |
| Disciplinas: | Biología |
| Palabras clave: | Angiospermas, Fisiología vegetal, Cultivo de tejidos, Embriogénesis somática, Reguladores del crecimiento, Callos, Plantas medicinales, Campomanesia adamantium, Myrtaceae |
| Keyword: | Angiosperms, Plant physiology, Tissue culture, Somatic embryogenesis, Growth regulators, Callus, Medicinal plants, Campomanesia adamantium, Myrtaceae |
| Texto completo: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46358/751375149052 |
