Validation of a method for simultaneous analysis of cocaine, benzoylecognine and cocaethylene in urine using gas chromatography-mass spectrometry
Título del documento: Validation of a method for simultaneous analysis of cocaine, benzoylecognine and cocaethylene in urine using gas chromatography-mass spectrometry
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451247
ISSN: 1984-8250
Autores: 1
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Instituciones: 1Universidade Estadual de Maringa, Programa de Pos-Graduacao em Biociencias e Fisiopatologia, Maringa, Parana. Brasil
2Universidade Estadual de Maringa, Departamento de Ciencias Basicas da Saude, Maringa, Parana. Brasil
3Universidade Estadual de Maringa, Programa de Pos-Graduacao em Enfermagem, Maringa, Parana. Brasil
Año:
Volumen: 56
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Aplicado, descriptivo
Resumen en inglés The present study describes a method for simultaneous analysis of cocaine, benzoylecgonine and cocaethylene in urine samples. After solid phase extraction, Gas Chromatography-Mass Spectrometry was used for identification and quantification. The calibration curves were linear at 20 - 3000 ng/mL, r2 0.9997 for benzoylecgonine, 15 - 2000 ng/mL, r2 0.9985 and r2 0.9993 for cocaine and cocaethylene, respectively. Accuracy values: cocaine: 93,5 - 102,1%; benzoylecgonine: 97,5 - 104,8%; cocaethylene: 90,6 - 101,5%. Precision values: cocaine: C.V 5,4 - 14,6%; benzoylecgonine: C.V 7,8 - 12,3%; cocaethylene: C.V 5,9 - 12,3%. Detection and quantification limit values: cocaine and cocaethylene:10 ng/mL and 15 ng/mL, respectivalty; benzoylecgonine:15 ng/mL and 20 ng/mL, respectivaley. Recovery values: cocaine: 78,0 - 85,8%; benzoylecgonine: 74,0 - 79,8%; cocaethylene: 83,0 - 91,5%. The method described is advantageous compared to others, as it simultaneously detects the major analytes found in urine samples due to cocaine use and has been successfully validated
Disciplinas: Medicina
Palabras clave: Toxicología,
Diagnóstico,
Análisis clínicos,
Validación analítica,
Orina,
Cromatografía de gases,
Espectrometría de masas,
Cocaína,
Benzoilecgonina
Keyword: Toxicology,
Diagnosis,
Clinical analysis,
Analytical validation,
Urine,
Gas chromatography,
Mass spectrometry,
Cocaine,
Benzoylecognine
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