Validation of a method for simultaneous analysis of cocaine, benzoylecognine and cocaethylene in urine using gas chromatography-mass spectrometry
Título del documento: Validation of a method for simultaneous analysis of cocaine, benzoylecognine and cocaethylene in urine using gas chromatography-mass spectrometry
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451247
ISSN: 1984-8250
Autors: 1
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Institucions: 1Universidade Estadual de Maringa, Programa de Pos-Graduacao em Biociencias e Fisiopatologia, Maringa, Parana. Brasil
2Universidade Estadual de Maringa, Departamento de Ciencias Basicas da Saude, Maringa, Parana. Brasil
3Universidade Estadual de Maringa, Programa de Pos-Graduacao em Enfermagem, Maringa, Parana. Brasil
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Volum: 56
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Aplicado, descriptivo
Resumen en inglés The present study describes a method for simultaneous analysis of cocaine, benzoylecgonine and cocaethylene in urine samples. After solid phase extraction, Gas Chromatography-Mass Spectrometry was used for identification and quantification. The calibration curves were linear at 20 - 3000 ng/mL, r2 0.9997 for benzoylecgonine, 15 - 2000 ng/mL, r2 0.9985 and r2 0.9993 for cocaine and cocaethylene, respectively. Accuracy values: cocaine: 93,5 - 102,1%; benzoylecgonine: 97,5 - 104,8%; cocaethylene: 90,6 - 101,5%. Precision values: cocaine: C.V 5,4 - 14,6%; benzoylecgonine: C.V 7,8 - 12,3%; cocaethylene: C.V 5,9 - 12,3%. Detection and quantification limit values: cocaine and cocaethylene:10 ng/mL and 15 ng/mL, respectivalty; benzoylecgonine:15 ng/mL and 20 ng/mL, respectivaley. Recovery values: cocaine: 78,0 - 85,8%; benzoylecgonine: 74,0 - 79,8%; cocaethylene: 83,0 - 91,5%. The method described is advantageous compared to others, as it simultaneously detects the major analytes found in urine samples due to cocaine use and has been successfully validated
Disciplines Medicina
Paraules clau: Toxicología,
Diagnóstico,
Análisis clínicos,
Validación analítica,
Orina,
Cromatografía de gases,
Espectrometría de masas,
Cocaína,
Benzoilecgonina
Keyword: Toxicology,
Diagnosis,
Clinical analysis,
Analytical validation,
Urine,
Gas chromatography,
Mass spectrometry,
Cocaine,
Benzoylecognine
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