Neutralization of the neuromuscular activity of bothropstoxin-i, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga)
Document title: Neutralization of the neuromuscular activity of bothropstoxin-i, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga)
Journal: The journal of venomous animals and toxins including tropical diseases
Database: PERIÓDICA
System number: 000298914
ISSN: 1678-9199
Authors: 1



2
3
Institutions: 1Universidade de Sorocaba, Faculdade de Farmacia, Sorocaba, Sao Paulo. Brasil
2Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil
3Universidade Estadual de Campinas, Departamento de Farmacologia, Campinas, Sao Paulo. Brasil
Year:
Season: Dic
Volumen: 11
Number: 4
Pages: 465-478
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Numerous plants are used as snakebite antidotes in Brazilian folk medicine, including Casearia sylvestris Swartz, popularly known as guaçatonga. In this study, we examined the action of a hydroalcoholic extract from C. sylvestris on the neuromuscular blockade caused by bothropstoxin-I (BthTX-I), a myotoxin from Bothrops jararacussu venom, in mouse isolated phrenic nerve-diaphragm (PND) preparations. Aqueous (8 and 12 mg/ml, n=4 and 5, respectively) and hydroalcoholic (12 mg/ml, n=12) extracts of the leaves of C. sylvestris caused facilitation in PND preparations followed by partial neuromuscular blockade. BthTX-I (20 µg/ml, n=4) caused 50% paralysis after 65±15 min (mean ± S.E.M). Preincubation (30 min at 37° C) of BthTX-I (20 µg/ml, n=4) with a concentration of the hydroalcoholic extract (4 mg/ml) that had no neuromuscular activity, such as the control (n=5), prevented the neuromuscular blockade caused by the toxin. This protection may be mediated by compounds such as flavonoids and phenols identified by thin-layer chromatography and colorimetric assays
Disciplines: Medicina
Keyword: Medicina alternativa,
Toxicología,
Mordeduras de serpiente,
Envenenamiento,
Fitoterapia,
Extractos vegetales,
Miotoxinas,
Neutralización,
Bothrops jararacussu,
Viperidae,
Casearia sylvestris,
Salicaceae
Keyword: Medicine,
Alternative medicine,
Toxicology,
Snake bites,
Envenomation,
Phytotherapy,
Plant extracts,
Myotoxins,
Neutralization,
Bothrops jararacussu,
Viperidae,
Casearia sylvestris,
Salicaceae
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