| Journal: | The journal of venomous animals and toxins including tropical diseases |
| Database: | PERIÓDICA |
| System number: | 000298795 |
| ISSN: | 1678-9199 |
| Authors: | Manzoli-Palma, M.F1 Gobbi, N Palma, M.S2 |
| Institutions: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Centro de Estudos Ambientais, Rio Claro, Sao Paulo. Brasil 2Universidade Estadual Paulista "Julio de Mesquita Filho", Centro de Estudos de Venenos e Animais Peconhentos, Botucatu, Sao Paulo. Brasil |
| Year: | 2003 |
| Volumen: | 9 |
| Number: | 2 |
| Pages: | 174-185 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, descriptivo |
| English abstract | This study was undertaken to develop an experimental protocol using insects as biological models to assay venom toxicity of the following spiders Loxosceles gaucho, Phoneutria nigriventer, Nephilengys cruentata and Tityus serrulatus scorpion. Three different insect species were bioassayed: Apis mellifera (Hymenoptera), Grillus assimilis (Orthoptera), and Diatraea saccharalis (Lepidoptera). Venoms were injected into the hemocele of insects with a microsyringe at concentrations that caused dose/weight-dependent effects; doses causing either paralysis (ED50) or death (LD50) were recorded for each venom and insect test-species. T. serrulatus and L. gaucho venoms were lethal to all tested species, while P. nigriventer venom caused paralysis and death, and N. cruentata venom caused only paralysis at the doses assayed. A comparison between the insect test species described above revealed that A. mellifera was highly sensitive to all venoms tested; even a tiny amount of N. cruentata non-lethal venom caused a change in the walking pattern leading to transient paralysis. D. saccharalis larvae were very resistant to all four venoms |
| Disciplines: | Biología |
| Keyword: | Insectos, Toxicología, Veneno de alacrán, Veneno de araña, Bioensayos, Toxicidad, Modelos animales |
| Keyword: | Biology, Insects, Toxicology, Scorpion venom, Spider venom, Bioassays, Toxicity, Animal models |
| Full text: | Texto completo (Ver HTML) |
