| Journal: | The journal of venomous animals and toxins including tropical diseases |
| Database: | PERIÓDICA |
| System number: | 000298832 |
| ISSN: | 1678-9199 |
| Authors: | Rahmy, T.R1 Hassona, I.A2 |
| Institutions: | 1Suez Channel University, Faculty of Science, Ismailia. Egipto 2United Arab Emirates University, Faculty of Science, Al-Ain. Emiratos Arabes Unidos |
| Year: | 2004 |
| Volumen: | 10 |
| Number: | 1 |
| Pages: | 53-76 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | The immunohistochemical expression of neuron-specific enolase, NSE (a cytoplasmic glycolytic enzyme of the neurons), synaptophysin, SYN (a major membrane glycoprotein of synaptic vesicles), and Bcl-2 (anti-apoptotic protein) were determined in cerebral cortex of rats envenomed with neurotoxic venom from Egyptian cobra. Male rats were intramuscularly (IM) injected with a single injection of either physiological saline solution or ½ LD50 or LD50 of cobra venom and sacrificed 24, 48, or 72 hr after envenoming. Formalin-fixed paraffin sections were immunohistochemically studied by avidin-biotin-peroxidase complex method. Neuron histological structure and isolation of genomic DNA were also detected. The results showed a dose and time-dependent increase in NSE and SYN immunoreactivity in cerebral cortex of envenomed rats except in 72 hr high dose envenoming, where decreased SYN was observed. On the other hand, low dose venom induced high Bcl-2 expression 24 hr after envenoming, while the high dose decreased Bcl-2 protein expression. Temporal and spatial Bcl-2 expression was accompanied by DNA fragmentation in cerebral cortex of all envenomed rats, although no serious histological alterations were noticed. These results suggest that cobra venom may lead to neuronal injury and impairment of axonal transport as ascertained by alterations in NSE and SYN immunoreactivity. It could also indicate that venom alters the molecular machinery of apoptosis by inhibiting Bcl-2 expression; however, some vulnerable cells have the ability to overcome thi |
| Disciplines: | Medicina |
| Keyword: | Medicina experimental, Neurología, Toxicología, Veneno de víbora, Daño neuronal, Enolasa, Sinaptofisina, Corteza cerebral, Inmunohistoquímica, Ratas |
| Keyword: | Medicine, Experimental medicine, Neurology, Toxicology, Snake venom, Neuronal damage, Enolase, Synaptophysin, Cerebral cortex, Immunohistochemistry, Rats |
| Full text: | Texto completo (Ver HTML) |
