| Journal: | The journal of venomous animals and toxins including tropical diseases |
| Database: | PERIÓDICA |
| System number: | 000298827 |
| ISSN: | 1678-9199 |
| Authors: | Netto, D.P1 Chiacchio, S.B2 Bicudo, P.L Alfieri, A.A Balarim, M.R.S Nascimento, N3 |
| Institutions: | 1Universidade Estadual de Londrina, Faculdade de Medicina Veterinaria e Zootecnia, Londrina, Parana. Brasil 2Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Medicina Veterinaria e Zootecnia, Botucatu, Sao Paulo. Brasil 3Comissao Nacional de Energia Nuclear, Instituto de Pesquisas Energeticas e Nucleares, Sao Paulo. Brasil |
| Year: | 2004 |
| Volumen: | 10 |
| Number: | 1 |
| Pages: | 34-52 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | Natural (NV) and Cobalto60-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC), total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD50 for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/ml indicates that slight leukocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in t |
| Disciplines: | Medicina veterinaria y zootecnia |
| Keyword: | Medicina veterinaria, Ovinos y caprinos, Hematología, Veneno de víbora, Antisueros, Ovejas, Crotalus durissus terrificus, Viperidae |
| Keyword: | Veterinary medicine and animal husbandry, Sheep and goats, Veterinary medicine, Hematology, Snake venom, Antiserum, Sheep, Crotalus durissus terrificus, Viperidae |
| Full text: | Texto completo (Ver HTML) |
