| Journal: | The journal of venomous animals and toxins including tropical diseases |
| Database: | PERIÓDICA |
| System number: | 000298837 |
| ISSN: | 1678-9199 |
| Authors: | Piatti, R.M1 Ikuno, A.A Baldassi, L |
| Institutions: | 1Instituto Biologico, Centro de Sanidade Animal, Sao Paulo. Brasil |
| Year: | 2004 |
| Volumen: | 10 |
| Number: | 2 |
| Pages: | 154-160 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types |
| Disciplines: | Biología |
| Keyword: | Bacterias, Microbiología, Alfa-toxina, Clostridium perfringens, Reacción en cadena de la polimerasa (PCR) |
| Keyword: | Biology, Bacteria, Microbiology, Alpha toxin, Clostridium perfringens, Polymerase chain reaction (PCR) |
| Full text: | Texto completo (Ver HTML) |
