| Journal: | The brazilian journal of infectious diseases |
| Database: | PERIÓDICA |
| System number: | 000290758 |
| ISSN: | 1413-8670 |
| Authors: | Silbert, Suzane1 Tosin, Iraci1 Sader, Helio S1 |
| Institutions: | 1Universidade Federal de Sao Paulo, Laboratorio Especial de Microbiologia Clinica, Sao Paulo. Brasil 2Universidade Federal de Santa Catarina, Centro de Ciencias Biologicas, Florianopolis, Santa Catarina. Brasil |
| Year: | 2003 |
| Season: | Dic |
| Volumen: | 7 |
| Number: | 6 |
| Pages: | 360-369 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Caso clínico, analítico |
| English abstract | Antimicrobial resistance has increased rapidly in Brazil and worldwide during the past few years, giving rise to a growing necessity for antimicrobial resistance surveillance programs. These programs have been instituted in order to monitor bacterial resistance in various regions, and to guide empirical antimicrobial therapy. We evaluated the use of molecular typing in multicenter surveillance programs. We also studied the dissemination modes of selected resistance profiles. Antimicrobial susceptibility to various antimicrobial agents was evaluated by the reference broth microdilution method. Bacterial isolates with selected susceptibility patterns were characterized by pulsed field-gel electrophoresis (PFGE). A total of 119 Gram-negative bacteria were molecularly typed, including 22 imipenem-resistant Pseudomonas aeruginosa, 26 ESBL-producing Escherichia coli, 27 cefoxitin-resistant-ESBL-producing Klebsiella pneumoniae, 33 Enterobacter spp., 8 Citrobacter spp., and 3 S. marcescens isolates resistant to ceftazidime. The isolates were from clinically apparent bacteremia of patients hospitalized in medical centers located in 13 cities of 11 Brazilian states. Our molecular typing results revealed a great genetic diversity among isolates of the same species. However, some major PFGE patterns were found in more than one isolate. All repeated PFGE patterns were detected in only 2 isolates, which were isolated within the same institutions or in different medical centers. We conclude that the ability to characterize organisms phenotypicall |
| Disciplines: | Química, Medicina |
| Keyword: | Bioquímica, Microbiología, Genética, Tipificación molecular, Electroforesis en gel de campo pulsado, Resistencia antimicrobiana, Carbapenemas, Pseudomonas aeruginosa, Difusión de clones, Programa centinela |
| Keyword: | Chemistry, Medicine, Biochemistry, Microbiology, Molecular typing, Pulsed field gel electrophoresis, Antimicrobial resistance, Carbapenems, Pseudomonas aeruginosa, Clonal dissemination, Sentinela Program, Genetics |
| Full text: | Texto completo (Ver HTML) |
