| Journal: | The brazilian journal of infectious diseases |
| Database: | PERIÓDICA |
| System number: | 000290984 |
| ISSN: | 1413-8670 |
| Authors: | Nomura, Marcelo Luis1 Passini-Junior, Renato Oliveira, Ulysses Moraes2 |
| Institutions: | 1Universidade Estadual de Campinas, Departamento de Obstetricia e Ginecologia, Campinas, Sao Paulo. Brasil 2Universidade Estadual de Campinas, Faculdade de Medicina, Campinas, Sao Paulo. Brasil |
| Year: | 2006 |
| Season: | Ago |
| Volumen: | 10 |
| Number: | 4 |
| Pages: | 247-250 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | The objective of this study was to identify group B streptococcus (GBS) colonization rates and compare detection efficiency of selective versus non-selective culture media and anorectal versus vaginal cultures in women with preterm labor and preterm-premature rupture of membranes (PROM). A prospective cohort study of 203 women was performed. Two vaginal and two anorectal samples from each woman were collected using sterile swabs. Two swabs (one anorectal and one vaginal) were placed separately in Stuart transport media and cultured in blood-agar plates for 48 hours; the other two swabs were inoculated separately in Todd-Hewitt selective media for 24 hours and then subcultured in blood-agar plates. Final GBS identification was made by the CAMP test. A hundred thrity-two cultures out of 812 were positive. The maternal colonization rate was 27.6%. Colonization rates were 30% for preterm PROM and 25.2% for preterm labor. Todd-Hewitt selective medium detected 87.5% and non-selective medium 60.7% GBS-positive women. Vaginal samples and anorectal samples had the same detection rate of 80.3%. Anorectal selective cultures detected 75% of carriers; 39% of GBS-positive women were detected only in selective medium. A combined vaginal-anorectal selective culture is appropriate for GBS screening in this population, minimizing laboratory costs |
| Disciplines: | Biología, Química, Medicina |
| Keyword: | Bacterias, Bioquímica, Ginecología y obstetricia, Streptococcus agalactiae, Medios de cultivo, Ruptura prematura de membranas, Parto prematuro |
| Keyword: | Biology, Chemistry, Medicine, Bacteria, Biochemistry, Gynecology and obstetrics, Streptococcus agalactiae, Culture media, Premature rupture of membranes, Preterm delivery |
| Full text: | Texto completo (Ver HTML) |
