| Journal: | Revista mexicana de ciencias forestales |
| Database: | PERIÓDICA |
| System number: | 000395644 |
| ISSN: | 2007-1132 |
| Authors: | Alvarez Moctezuma, José Guadalupe1 Ortiz Reyes, Alma Delia1 Sahagún Castellanos, Jaime1 Peña Lomelí, Aureliano1 |
| Institutions: | 1Universidad Autónoma Chapingo, Departamento de Fitotecnia, Chapingo, Estado de México. México |
| Year: | 2012 |
| Season: | Jul-Ago |
| Volumen: | 3 |
| Number: | 12 |
| Country: | México |
| Language: | Español |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| Spanish abstract | Abies religiosa tiene gran potencial para utilizarse como árbol urbano, árbol de Navidad, bonsai, para obtención de madera y celulosa, construcción de techos, medicina y para secuestro de carbono. Sus plantaciones requieren de cobertura arbórea, porque es tolerante a la sombra y sus brinzales son susceptibles a la intensidad luminosa alta. Aunque, muestra variación intraespecífica natural y variación fenotípica interespecífica, a esta variable. El objetivo del presente estudio fue detectar el perfil RAPD asociado con la resistencia a la intensidad luminosa alta en brinzales de A. religiosa. Se aisló ADN de 15 brinzales resistentes y 15 susceptibles a dicha condición, y se evaluaron 35 iniciadores en dos muestras compuestas. De aquellos con productos de PCR, se realizó una segunda reacción con ADN de brinzales individuales. Las variables consideradas fueron supervivencia del brinzal a la intensidad luminosa alta, número de hojas, apertura estomatal, número de estomas y altura del brinzal. Se calcularon las correlaciones entre esas variables y los perfiles de RAPD. Se detectaron bandas que correlacionaron con las variables. La supervivencia de los brinzales alcanzó un valor de 24 %. El ancho y apertura estomatal tuvieron mayor cantidad de correlaciones. La banda 1801.92 pb del iniciador 5'-CCGGCCTTCC-3' se correlacionó con casi todas las variables. La supervivencia está determinada, principalmente, por la apertura de los estomas; estas, así como la altura y el número de hojas están controladas genéticamente |
| English abstract | Abies religiosa has great potential as urban forest tree, Christmas-tree, bonsai, timber, paper, ceiling, medicinal, and carbon sequestration. However, plantations are not possible without tree covering, because this species is shade tolerant, and their seedlings are susceptible to high intensity light. Nevertheless, A. religiosa shows natural intraspecific variation in resistance to high intensity light. Because of this and because exist interspecific phenotipic variation for resistance to high intensity light. The probability that there is a genetic basis for the intraspecific variation must be high. The aim of this study was to detect RAPD profiles associated with resistance to high intensity light on A. religiosa seedlings. The DNA from 15 resistant and 15 susceptible seedlings to high intensity light were isolated. Two bulked samples were formed and 35 primers were evaluated. From primers with PCR products, a second PCR reaction with DNA from individual seedlings was done. Registered data were: seedling survival to high intensity light, leaf number, stomata aperture, stomata number, and plant height. Correlations between these variables, and RAPDs profiles for each primer were computed. Bands which correlated with all variables were detected. 24 % of the seedlings survived to high intensity light. Stomata width and aperture showed the largest number of correlations. The 1801.92 pb band from primer 5'-CCGGCCTTCC-3' was correlated with almost all variables. This suggests that survival, height, leaf number, and stomata aperture are genetically controlled variables. Seedling survival to high intensity light is mainly determined by stomata aperture |
| Disciplines: | Agrociencias, Biología |
| Keyword: | Silvicultura, Fisiología vegetal, Abies religiosa, Luz, Marcadores moleculares, RAPD, Estomas, Resistencia |
| Keyword: | Agricultural sciences, Biology, Silviculture, Plant physiology, Abies religiosa, Light, Molecular markers, RAPD, Stomata, Resistance |
| Full text: | Texto completo (Ver HTML) |
