Journal: | Revista do Instituto de Medicina Tropical de Sao Paulo |
Database: | |
System number: | 000537080 |
ISSN: | 0036-4665 |
Authors: | Cadore, Priscila Silva1 Walcher, Débora Liliane2 Sousa, Nicholas Frota Gonçalves Correia de3 Martins, Lourdes Helena Rodrigues1 Hora, Vanusa Pousada da1 Groll, Andrea Von1 Moura, Micaele Quintana de1 Berne, Maria Elisabeth Aires2 Avila, Luciana Farias da Costa de1 Scaini, Carlos James1 |
Institutions: | 1Universidade Federal do Rio Grande, Hospital Universitário, Rio Grande, Rio Grande do Sul. Brasil 2Universidade Federal de Pelotas, Departamento de Microbiologia e Parasitologia, Pelotas, Rio Grande do Sul. Brasil 3Universidade Federal do Rio Grande, Faculdade de Medicina Rio Grande, Rio Grande do Sul. Brasil |
Year: | 2021 |
Volumen: | 63 |
Country: | Brasil |
Language: | Inglés |
English abstract | Human toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ cytokine on parasite-host in vivo (1.109 CFU) and in vitro (1.106, 1.107, 1.108, 1.109 CFU) interactions. Four groups of six BALB/c mice were formed: G1 - L. acidophilus supplementation and T. canis infection; G2 - T. canis infection; G3 - L. acidophilus supplementation; and G4 - PBS administration. Mice were intragastrically suplemented with probiotics for 15 days before inoculation and 48 h after inoculation with 100 T. canis eggs. The inoculation of T. canis was also perfomed intragastrically. The recovery of larvae took place through digestion of liver and lung tissues; the evaluation of IFN-γ gene transcription in leukocytes was performed by qPCR. The in vitro test consisted of incubating the probiotic with T. canis larvae. The supplementation of probiotics produced a reduction of 57.7% (p = 0.025) in the intensity of infection of T. canis larvae in mice, whereas in the in vitro test, there was no larvicidal effect. In addition, a decrease in the IFN-γ gene transcription was observed in both, T. canis-infected and uninfected mice, regardless of whether or not they received supplementation. The probiotic L. acidophilus ATCC 4356 reduced T. canis infection intensity in mice, however, the probiotic did not have a direct effect on larvae, demonstrating the need of interaction with the host for the beneficial effect of the probiotic to occur. Yet, the proinflammatory cytokine IFN-γ did not apparently contributed to the observed beneficial effect of probiotics. |
Keyword: | Toxocariasis, Probiotic, Innate immunity, Cytokine, INF-γ |
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