| Journal: | Revista do Instituto de Medicina Tropical de Sao Paulo |
| Database: | |
| System number: | 000547886 |
| ISSN: | 0036-4665 |
| Authors: | Alarcon Valdes, Patricia1 Sanchez Aguillon, Fabiola2 Martinez Hernandez, Fernando3 Olivo Diaz, Angelica2 Maravilla, Pablo3 Santillan Benitez, Jonnathan Guadalupe1 Romero Valdovinos, Mirza2 |
| Institutions: | 1Universidad Autónoma del Estado de México, Departmento de Biologia Molecular, Toluca, Estado de México. México 2Hospital General Dr. Manuel Gea Gonzalez, Departmento de Biologia Molecular e Histocompatibilidad, Ciudad de Mexico. México 3Hospital General Dr. Manuel Gea Gonzalez, Departmento de Ecologia de Agentes Patogenos, Ciudad de Mexico. México |
| Year: | 2022 |
| Volumen: | 64 |
| Country: | Brasil |
| Language: | Inglés |
| English abstract | Human Adenovirus 36 (HAdV-36) has been related to diverse effects on metabolism and may attenuate the lipid accumulation in kidneys with increased adiposity. Some of these effects would be related to viral persistence. However, until now, a model of persistent in vitro infection by HAdV-36 is unknown. In this study, we examined the cells of the Vero lineage to explore their permissiveness to long-term HAdV-36 infection. HAdV-36 was productively replicated in Vero cells and maintained long-term infection for up to 35 cell passages. A subculture was obtained from the cells that survived the primary infection at a low MOI (0.5). The production of the extracellular infectious virus with titers ranging from 104 to 106 TCID50/mL and DNA-bearing cells was detected. In long-term infected cells, the intracellular distribution of viral antigen was demonstrated by performing immunolocalization (IFI) and expression of cell-viral antigen in 50% of cells by flow cytometry, using anti-HAdV-36 hyperimmune rabbit serum. Furthermore, E1a and E4orf1 genes in long-term infected passages showed a decreasing trend. Our preliminary results reveal that renal epithelial monkey cells are permissive for the productive infection of HAdV-36. Vero cell culture long-term infection might be a promising model for addressing the fundamental aspects of the HAdV-36 biology that cannot reveal broadly-used cultures, which do not maintain long-term infection in primary or transformed cells. |
| Keyword: | HAdV-36, Ad36, Vero cells, HAdV-36 long-term in vitro infection |
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